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Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respective...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211696/ https://www.ncbi.nlm.nih.gov/pubmed/34140582 http://dx.doi.org/10.1038/s41598-021-92179-y |
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author | Malheiros, Jessica Moraes Correia, Banny Silva Barbosa Ceribeli, Caroline Cardoso, Daniel Rodrigues Colnago, Luiz Alberto Junior, Stanislau Bogusz Reecy, James Mark Mourão, Gerson Barreto Coutinho, Luiz Lehmann Palhares, Julio Cesar Pascale Berndt, Alexandre de Almeida Regitano, Luciana Correia |
author_facet | Malheiros, Jessica Moraes Correia, Banny Silva Barbosa Ceribeli, Caroline Cardoso, Daniel Rodrigues Colnago, Luiz Alberto Junior, Stanislau Bogusz Reecy, James Mark Mourão, Gerson Barreto Coutinho, Luiz Lehmann Palhares, Julio Cesar Pascale Berndt, Alexandre de Almeida Regitano, Luciana Correia |
author_sort | Malheiros, Jessica Moraes |
collection | PubMed |
description | We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines. |
format | Online Article Text |
id | pubmed-8211696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82116962021-06-21 Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) Malheiros, Jessica Moraes Correia, Banny Silva Barbosa Ceribeli, Caroline Cardoso, Daniel Rodrigues Colnago, Luiz Alberto Junior, Stanislau Bogusz Reecy, James Mark Mourão, Gerson Barreto Coutinho, Luiz Lehmann Palhares, Julio Cesar Pascale Berndt, Alexandre de Almeida Regitano, Luciana Correia Sci Rep Article We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines. Nature Publishing Group UK 2021-06-17 /pmc/articles/PMC8211696/ /pubmed/34140582 http://dx.doi.org/10.1038/s41598-021-92179-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Malheiros, Jessica Moraes Correia, Banny Silva Barbosa Ceribeli, Caroline Cardoso, Daniel Rodrigues Colnago, Luiz Alberto Junior, Stanislau Bogusz Reecy, James Mark Mourão, Gerson Barreto Coutinho, Luiz Lehmann Palhares, Julio Cesar Pascale Berndt, Alexandre de Almeida Regitano, Luciana Correia Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) |
title | Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) |
title_full | Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) |
title_fullStr | Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) |
title_full_unstemmed | Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) |
title_short | Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) |
title_sort | comparative untargeted metabolome analysis of ruminal fluid and feces of nelore steers (bos indicus) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211696/ https://www.ncbi.nlm.nih.gov/pubmed/34140582 http://dx.doi.org/10.1038/s41598-021-92179-y |
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