Cargando…

Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)

We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respective...

Descripción completa

Detalles Bibliográficos
Autores principales: Malheiros, Jessica Moraes, Correia, Banny Silva Barbosa, Ceribeli, Caroline, Cardoso, Daniel Rodrigues, Colnago, Luiz Alberto, Junior, Stanislau Bogusz, Reecy, James Mark, Mourão, Gerson Barreto, Coutinho, Luiz Lehmann, Palhares, Julio Cesar Pascale, Berndt, Alexandre, de Almeida Regitano, Luciana Correia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211696/
https://www.ncbi.nlm.nih.gov/pubmed/34140582
http://dx.doi.org/10.1038/s41598-021-92179-y
_version_ 1783709520772464640
author Malheiros, Jessica Moraes
Correia, Banny Silva Barbosa
Ceribeli, Caroline
Cardoso, Daniel Rodrigues
Colnago, Luiz Alberto
Junior, Stanislau Bogusz
Reecy, James Mark
Mourão, Gerson Barreto
Coutinho, Luiz Lehmann
Palhares, Julio Cesar Pascale
Berndt, Alexandre
de Almeida Regitano, Luciana Correia
author_facet Malheiros, Jessica Moraes
Correia, Banny Silva Barbosa
Ceribeli, Caroline
Cardoso, Daniel Rodrigues
Colnago, Luiz Alberto
Junior, Stanislau Bogusz
Reecy, James Mark
Mourão, Gerson Barreto
Coutinho, Luiz Lehmann
Palhares, Julio Cesar Pascale
Berndt, Alexandre
de Almeida Regitano, Luciana Correia
author_sort Malheiros, Jessica Moraes
collection PubMed
description We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines.
format Online
Article
Text
id pubmed-8211696
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-82116962021-06-21 Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus) Malheiros, Jessica Moraes Correia, Banny Silva Barbosa Ceribeli, Caroline Cardoso, Daniel Rodrigues Colnago, Luiz Alberto Junior, Stanislau Bogusz Reecy, James Mark Mourão, Gerson Barreto Coutinho, Luiz Lehmann Palhares, Julio Cesar Pascale Berndt, Alexandre de Almeida Regitano, Luciana Correia Sci Rep Article We conducted a study to identify the fecal metabolite profile and its proximity to the ruminal metabolism of Nelore steers based on an untargeted metabolomic approach. Twenty-six Nelore were feedlot with same diet during 105 d. Feces and rumen fluid were collected before and at slaughter, respectively. The metabolomics analysis indicated 49 common polar metabolites in the rumen and feces. Acetate, propionate, and butyrate were the most abundant polar metabolites in both bio-samples. The rumen presented significantly higher concentrations of the polar compounds when compared to feces (P < 0.05); even though, fecal metabolites presented an accentuated representability of the ruminal fluid metabolites. All fatty acids present in the ruminal fluid were also observed in the feces, except for C20:2n6 and C20:4n6. The identified metabolites offer information on the main metabolic pathways (higher impact factor and P < 0.05), as synthesis and degradation of ketone bodies; the alanine, aspartate and glutamate metabolisms, the glycine, serine; and threonine metabolism and the pyruvate metabolism. The findings reported herein on the close relationship between the ruminal fluid and feces metabolic profiles may offer new metabolic information, in addition to facilitating the sampling for metabolism investigation in animal production and health routines. Nature Publishing Group UK 2021-06-17 /pmc/articles/PMC8211696/ /pubmed/34140582 http://dx.doi.org/10.1038/s41598-021-92179-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Malheiros, Jessica Moraes
Correia, Banny Silva Barbosa
Ceribeli, Caroline
Cardoso, Daniel Rodrigues
Colnago, Luiz Alberto
Junior, Stanislau Bogusz
Reecy, James Mark
Mourão, Gerson Barreto
Coutinho, Luiz Lehmann
Palhares, Julio Cesar Pascale
Berndt, Alexandre
de Almeida Regitano, Luciana Correia
Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_full Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_fullStr Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_full_unstemmed Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_short Comparative untargeted metabolome analysis of ruminal fluid and feces of Nelore steers (Bos indicus)
title_sort comparative untargeted metabolome analysis of ruminal fluid and feces of nelore steers (bos indicus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211696/
https://www.ncbi.nlm.nih.gov/pubmed/34140582
http://dx.doi.org/10.1038/s41598-021-92179-y
work_keys_str_mv AT malheirosjessicamoraes comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT correiabannysilvabarbosa comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT ceribelicaroline comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT cardosodanielrodrigues comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT colnagoluizalberto comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT juniorstanislaubogusz comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT reecyjamesmark comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT mouraogersonbarreto comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT coutinholuizlehmann comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT palharesjuliocesarpascale comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT berndtalexandre comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus
AT dealmeidaregitanolucianacorreia comparativeuntargetedmetabolomeanalysisofruminalfluidandfecesofneloresteersbosindicus