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Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing
It is commonly observed that patients with bone fracture concomitant with traumatic brain injury (TBI) had significantly increased fracture healing, but the underlying mechanisms were not fully revealed. Long non-coding RNAs (lncRNAs) are known to play complicated roles in bone homeostasis, but thei...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211774/ https://www.ncbi.nlm.nih.gov/pubmed/34150839 http://dx.doi.org/10.3389/fsurg.2021.663377 |
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author | Guo, Guoning Gou, Yajun Jiang, Xingyu Wang, Shuhong Wang, Ruilie Liang, Changqiang Yang, Guang Wang, Tinggang Yu, Anyong Zhu, Guoyan |
author_facet | Guo, Guoning Gou, Yajun Jiang, Xingyu Wang, Shuhong Wang, Ruilie Liang, Changqiang Yang, Guang Wang, Tinggang Yu, Anyong Zhu, Guoyan |
author_sort | Guo, Guoning |
collection | PubMed |
description | It is commonly observed that patients with bone fracture concomitant with traumatic brain injury (TBI) had significantly increased fracture healing, but the underlying mechanisms were not fully revealed. Long non-coding RNAs (lncRNAs) are known to play complicated roles in bone homeostasis, but their role in TBI accelerated fracture was rarely reported. The present study was designed to determine the role of lncRNAs in TBI accelerated fracture via transcriptome sequencing and further bioinformatics analyses. Blood samples from three fracture-only patients, three fracture concomitant with TBI patients, and three healthy controls were harvested and were subsequently subjected to transcriptome lncRNA sequencing. Differentially expressed genes were identified, and pathway enrichment was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. High-dimensional data visualization by self-organizing map (SOM) machine learning was applied to further interpret the data. An xCell method was then used to predict cellular behavior in all samples based on gene expression profiles, and an lncRNA–cell interaction network was generated. A total of 874 differentially expressed genes were identified, of which about 26% were lncRNAs. Those identified lncRNAs were mainly enriched on TBI-related and damage repair-related pathways. SOM analyses revealed that those differentially expressed lncRNAs could be divided into three major module implications and were mainly enriched on transcriptional regulation and immune-related signal pathways, which promote us to further explore cellular behaviors based on differentially expressed lncRNAs. We have predicted that basophils, CD8+ T effector memory cells, B cells, and naïve B cells were significantly downregulated, while microvascular endothelial cells were predicted to be significantly upregulated in the Fr/TBI group, was the lowest and highest, respectively. ENSG00000278905, ENSG00000240980, ENSG00000255670, and ENSG00000196634 were the most differentially expressed lncRNAs related to all changes of cellular behavior. The present study has revealed for the first time that several critical lncRNAs may participate in TBI accelerated fracture potentially via regulating cellular behaviors of basophils, cytotoxic T cells, B cells, and endothelial cells. |
format | Online Article Text |
id | pubmed-8211774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82117742021-06-19 Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing Guo, Guoning Gou, Yajun Jiang, Xingyu Wang, Shuhong Wang, Ruilie Liang, Changqiang Yang, Guang Wang, Tinggang Yu, Anyong Zhu, Guoyan Front Surg Surgery It is commonly observed that patients with bone fracture concomitant with traumatic brain injury (TBI) had significantly increased fracture healing, but the underlying mechanisms were not fully revealed. Long non-coding RNAs (lncRNAs) are known to play complicated roles in bone homeostasis, but their role in TBI accelerated fracture was rarely reported. The present study was designed to determine the role of lncRNAs in TBI accelerated fracture via transcriptome sequencing and further bioinformatics analyses. Blood samples from three fracture-only patients, three fracture concomitant with TBI patients, and three healthy controls were harvested and were subsequently subjected to transcriptome lncRNA sequencing. Differentially expressed genes were identified, and pathway enrichment was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. High-dimensional data visualization by self-organizing map (SOM) machine learning was applied to further interpret the data. An xCell method was then used to predict cellular behavior in all samples based on gene expression profiles, and an lncRNA–cell interaction network was generated. A total of 874 differentially expressed genes were identified, of which about 26% were lncRNAs. Those identified lncRNAs were mainly enriched on TBI-related and damage repair-related pathways. SOM analyses revealed that those differentially expressed lncRNAs could be divided into three major module implications and were mainly enriched on transcriptional regulation and immune-related signal pathways, which promote us to further explore cellular behaviors based on differentially expressed lncRNAs. We have predicted that basophils, CD8+ T effector memory cells, B cells, and naïve B cells were significantly downregulated, while microvascular endothelial cells were predicted to be significantly upregulated in the Fr/TBI group, was the lowest and highest, respectively. ENSG00000278905, ENSG00000240980, ENSG00000255670, and ENSG00000196634 were the most differentially expressed lncRNAs related to all changes of cellular behavior. The present study has revealed for the first time that several critical lncRNAs may participate in TBI accelerated fracture potentially via regulating cellular behaviors of basophils, cytotoxic T cells, B cells, and endothelial cells. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8211774/ /pubmed/34150839 http://dx.doi.org/10.3389/fsurg.2021.663377 Text en Copyright © 2021 Guo, Gou, Jiang, Wang, Wang, Liang, Yang, Wang, Yu and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Surgery Guo, Guoning Gou, Yajun Jiang, Xingyu Wang, Shuhong Wang, Ruilie Liang, Changqiang Yang, Guang Wang, Tinggang Yu, Anyong Zhu, Guoyan Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing |
title | Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing |
title_full | Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing |
title_fullStr | Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing |
title_full_unstemmed | Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing |
title_short | Long Non-coding RNAs in Traumatic Brain Injury Accelerated Fracture Healing |
title_sort | long non-coding rnas in traumatic brain injury accelerated fracture healing |
topic | Surgery |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211774/ https://www.ncbi.nlm.nih.gov/pubmed/34150839 http://dx.doi.org/10.3389/fsurg.2021.663377 |
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