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Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma

Tumor-targeting peptides functioned as molecular probes are essential for multi-modality imaging and molecular-targeting therapy in caner theronostics. Here, we performed a phage-displayed bio-panning to identify a specific binding peptide targeting Glypican-3 (GPC-3), a promising biomarker in hepat...

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Autores principales: Yan, Jiayao, Yu, Xiaoxiao, Chen, Xiaotong, Liu, Fangcen, Chen, Fangjun, Ding, Naiqing, Yu, Lixia, Meng, Fanyan, Shen, Jie, Wei, Jia, Liu, Baorui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212053/
https://www.ncbi.nlm.nih.gov/pubmed/34150644
http://dx.doi.org/10.3389/fonc.2021.679336
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author Yan, Jiayao
Yu, Xiaoxiao
Chen, Xiaotong
Liu, Fangcen
Chen, Fangjun
Ding, Naiqing
Yu, Lixia
Meng, Fanyan
Shen, Jie
Wei, Jia
Liu, Baorui
author_facet Yan, Jiayao
Yu, Xiaoxiao
Chen, Xiaotong
Liu, Fangcen
Chen, Fangjun
Ding, Naiqing
Yu, Lixia
Meng, Fanyan
Shen, Jie
Wei, Jia
Liu, Baorui
author_sort Yan, Jiayao
collection PubMed
description Tumor-targeting peptides functioned as molecular probes are essential for multi-modality imaging and molecular-targeting therapy in caner theronostics. Here, we performed a phage-displayed bio-panning to identify a specific binding peptide targeting Glypican-3 (GPC-3), a promising biomarker in hepatocellular carcinoma (HCC). After screening in the cyclic peptide library, a candidate peptide named F3, was isolated and showed specific binding to HCC cell lines. In a bio-distribution study, higher accumulation of F3 peptide was observed in HepG-2 tumors compared to PC-3 tumors in xenograft models. After labeling with radioactive (68)Ga, the F3 peptide tracer enabled the specific detection of tumors in HCC tumor models with PET imaging. More importantly, the expression of GPC-3 in human tissue samples may be distinguished by an F3 fluorescent peptide probe indicating its potential for clinical application. This cyclic peptide targeting GPC-3 has been validated, and may be an alternative to serve as an imaging probe or a targeting domain in the drug conjugate.
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spelling pubmed-82120532021-06-19 Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma Yan, Jiayao Yu, Xiaoxiao Chen, Xiaotong Liu, Fangcen Chen, Fangjun Ding, Naiqing Yu, Lixia Meng, Fanyan Shen, Jie Wei, Jia Liu, Baorui Front Oncol Oncology Tumor-targeting peptides functioned as molecular probes are essential for multi-modality imaging and molecular-targeting therapy in caner theronostics. Here, we performed a phage-displayed bio-panning to identify a specific binding peptide targeting Glypican-3 (GPC-3), a promising biomarker in hepatocellular carcinoma (HCC). After screening in the cyclic peptide library, a candidate peptide named F3, was isolated and showed specific binding to HCC cell lines. In a bio-distribution study, higher accumulation of F3 peptide was observed in HepG-2 tumors compared to PC-3 tumors in xenograft models. After labeling with radioactive (68)Ga, the F3 peptide tracer enabled the specific detection of tumors in HCC tumor models with PET imaging. More importantly, the expression of GPC-3 in human tissue samples may be distinguished by an F3 fluorescent peptide probe indicating its potential for clinical application. This cyclic peptide targeting GPC-3 has been validated, and may be an alternative to serve as an imaging probe or a targeting domain in the drug conjugate. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212053/ /pubmed/34150644 http://dx.doi.org/10.3389/fonc.2021.679336 Text en Copyright © 2021 Yan, Yu, Chen, Liu, Chen, Ding, Yu, Meng, Shen, Wei and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Yan, Jiayao
Yu, Xiaoxiao
Chen, Xiaotong
Liu, Fangcen
Chen, Fangjun
Ding, Naiqing
Yu, Lixia
Meng, Fanyan
Shen, Jie
Wei, Jia
Liu, Baorui
Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma
title Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma
title_full Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma
title_fullStr Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma
title_full_unstemmed Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma
title_short Identification of a Glypican-3 Binding Peptide From a Phage-Displayed Peptide Library for PET Imaging of Hepatocellular Carcinoma
title_sort identification of a glypican-3 binding peptide from a phage-displayed peptide library for pet imaging of hepatocellular carcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212053/
https://www.ncbi.nlm.nih.gov/pubmed/34150644
http://dx.doi.org/10.3389/fonc.2021.679336
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