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Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function

Copper (Cu) is an essential trace element for animals, and also an important nutritional component for the normal physiology and metabolism of animal reproductive systems. An excess or lack of Cu will directly or indirectly affect animal reproductive activities. However, the effect of Cu, in particu...

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Autores principales: Chen, Jingyue, Cui, Zhaokang, Qiu, Yawei, Zhang, Xingxing, Chen, Fang, Wang, Huili, Xiong, Bo, Miao, Yilong, Gao, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212058/
https://www.ncbi.nlm.nih.gov/pubmed/34150773
http://dx.doi.org/10.3389/fcell.2021.678665
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author Chen, Jingyue
Cui, Zhaokang
Qiu, Yawei
Zhang, Xingxing
Chen, Fang
Wang, Huili
Xiong, Bo
Miao, Yilong
Gao, Qian
author_facet Chen, Jingyue
Cui, Zhaokang
Qiu, Yawei
Zhang, Xingxing
Chen, Fang
Wang, Huili
Xiong, Bo
Miao, Yilong
Gao, Qian
author_sort Chen, Jingyue
collection PubMed
description Copper (Cu) is an essential trace element for animals, and also an important nutritional component for the normal physiology and metabolism of animal reproductive systems. An excess or lack of Cu will directly or indirectly affect animal reproductive activities. However, the effect of Cu, in particular excessive Cu, on the reproductive performance of sows has not been studied. Here, we report that excessive Cu had negative effects on oocyte maturation and organelle functions. We showed that Cu exposure perturbed porcine oocyte meiotic maturation and impaired spindle/chromosome structure, resulting in a defective spindle assembly, as well as the abnormal distribution of actin dynamics and cortical granules. In addition, single-cell transcriptome analysis identified the target effectors of Cu actions in porcine oocytes, further demonstrating that Cu exposure affects the mitochondrial distribution and function, leading to the high levels of reactive oxygen species, DNA damage, and early apoptosis of porcine oocytes. These findings demonstrate that Cu exposure causes abnormalities in the mitochondrial distribution and function, resulting in the increased oxidative stress and levels of reactive oxygen species, DNA damage, and apoptosis, ultimately leading to a decreased porcine oocyte quality.
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spelling pubmed-82120582021-06-19 Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function Chen, Jingyue Cui, Zhaokang Qiu, Yawei Zhang, Xingxing Chen, Fang Wang, Huili Xiong, Bo Miao, Yilong Gao, Qian Front Cell Dev Biol Cell and Developmental Biology Copper (Cu) is an essential trace element for animals, and also an important nutritional component for the normal physiology and metabolism of animal reproductive systems. An excess or lack of Cu will directly or indirectly affect animal reproductive activities. However, the effect of Cu, in particular excessive Cu, on the reproductive performance of sows has not been studied. Here, we report that excessive Cu had negative effects on oocyte maturation and organelle functions. We showed that Cu exposure perturbed porcine oocyte meiotic maturation and impaired spindle/chromosome structure, resulting in a defective spindle assembly, as well as the abnormal distribution of actin dynamics and cortical granules. In addition, single-cell transcriptome analysis identified the target effectors of Cu actions in porcine oocytes, further demonstrating that Cu exposure affects the mitochondrial distribution and function, leading to the high levels of reactive oxygen species, DNA damage, and early apoptosis of porcine oocytes. These findings demonstrate that Cu exposure causes abnormalities in the mitochondrial distribution and function, resulting in the increased oxidative stress and levels of reactive oxygen species, DNA damage, and apoptosis, ultimately leading to a decreased porcine oocyte quality. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212058/ /pubmed/34150773 http://dx.doi.org/10.3389/fcell.2021.678665 Text en Copyright © 2021 Chen, Cui, Qiu, Zhang, Chen, Wang, Xiong, Miao and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Chen, Jingyue
Cui, Zhaokang
Qiu, Yawei
Zhang, Xingxing
Chen, Fang
Wang, Huili
Xiong, Bo
Miao, Yilong
Gao, Qian
Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function
title Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function
title_full Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function
title_fullStr Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function
title_full_unstemmed Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function
title_short Exposure to Copper Compromises the Maturational Competency of Porcine Oocytes by Impairing Mitochondrial Function
title_sort exposure to copper compromises the maturational competency of porcine oocytes by impairing mitochondrial function
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212058/
https://www.ncbi.nlm.nih.gov/pubmed/34150773
http://dx.doi.org/10.3389/fcell.2021.678665
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