A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing...

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Autores principales: Shi, Gongyao, Hao, Mengyuan, Tian, Baoming, Cao, Gangqiang, Wei, Fang, Xie, Zhengqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212136/
https://www.ncbi.nlm.nih.gov/pubmed/34149770
http://dx.doi.org/10.3389/fpls.2021.671091
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author Shi, Gongyao
Hao, Mengyuan
Tian, Baoming
Cao, Gangqiang
Wei, Fang
Xie, Zhengqing
author_facet Shi, Gongyao
Hao, Mengyuan
Tian, Baoming
Cao, Gangqiang
Wei, Fang
Xie, Zhengqing
author_sort Shi, Gongyao
collection PubMed
description As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.
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spelling pubmed-82121362021-06-19 A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants Shi, Gongyao Hao, Mengyuan Tian, Baoming Cao, Gangqiang Wei, Fang Xie, Zhengqing Front Plant Sci Plant Science As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212136/ /pubmed/34149770 http://dx.doi.org/10.3389/fpls.2021.671091 Text en Copyright © 2021 Shi, Hao, Tian, Cao, Wei and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shi, Gongyao
Hao, Mengyuan
Tian, Baoming
Cao, Gangqiang
Wei, Fang
Xie, Zhengqing
A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
title A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
title_full A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
title_fullStr A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
title_full_unstemmed A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
title_short A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants
title_sort methodological advance of tobacco rattle virus-induced gene silencing for functional genomics in plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212136/
https://www.ncbi.nlm.nih.gov/pubmed/34149770
http://dx.doi.org/10.3389/fpls.2021.671091
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