Cargando…

De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)

The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Zhili, Zhang, Wenyu, Liu, Yang, Li, Shuai, Yao, Wu, Sun, Xiaohui, Li, Siyu, Ma, Lichao, Sun, Juan, Yang, Qingchuan, Li, Yongxiang, Yang, Guofeng, Wang, Zeng-Yu, Cong, Lili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer India 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212189/
https://www.ncbi.nlm.nih.gov/pubmed/34220046
http://dx.doi.org/10.1007/s12298-021-00995-3
_version_ 1783709618447319040
author Zhao, Zhili
Zhang, Wenyu
Liu, Yang
Li, Shuai
Yao, Wu
Sun, Xiaohui
Li, Siyu
Ma, Lichao
Sun, Juan
Yang, Qingchuan
Li, Yongxiang
Yang, Guofeng
Wang, Zeng-Yu
Cong, Lili
author_facet Zhao, Zhili
Zhang, Wenyu
Liu, Yang
Li, Shuai
Yao, Wu
Sun, Xiaohui
Li, Siyu
Ma, Lichao
Sun, Juan
Yang, Qingchuan
Li, Yongxiang
Yang, Guofeng
Wang, Zeng-Yu
Cong, Lili
author_sort Zhao, Zhili
collection PubMed
description The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore, maintaining excellent traits through seed reproduction is a prime challenge in alfalfa. However, the cutting propagation technology could enable consistent multiplication of quality plants that are genetically identical to the parent plant. The current study aimed to develop a simple, cost-effective, reproducible, and efficient hydroponic cutting method to preserve alfalfa plants and for molecular research. In this study, alfalfa landrace ‘Wudi’ was grown in hydroponics for 30 days and used as source material for cuttings. The top, middle and bottom sections of its stem were used as cuttings. The rooting rate, root length, and stem height of the different stem sections were compared to determine the best segment for alfalfa propagation in four nutrient treatments (H(M), H(M) + 1/500H, H(M) + 1/1000H and d H(M) + 1/2000H). After 21 days of culture, the rooting rates of all the three stem types under four cutting nutrient solutions were above 78%. The rooting rate of the middle and bottom parts in H(M) + 1/1000 H and H(M) + 1/2000 H nutrient solutions reached more than 93%, with a higher health survey score (> 4.70). In conclusion, this study developed a de novo cutting propagation method that can be used to conserve and propagate germplasm in breeding programs and research. This method is a new report on the cutting propagation of alfalfa by hydroponics, which could supplement the existing cutting propagation methods.
format Online
Article
Text
id pubmed-8212189
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer India
record_format MEDLINE/PubMed
spelling pubmed-82121892021-07-01 De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) Zhao, Zhili Zhang, Wenyu Liu, Yang Li, Shuai Yao, Wu Sun, Xiaohui Li, Siyu Ma, Lichao Sun, Juan Yang, Qingchuan Li, Yongxiang Yang, Guofeng Wang, Zeng-Yu Cong, Lili Physiol Mol Biol Plants Research Article The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore, maintaining excellent traits through seed reproduction is a prime challenge in alfalfa. However, the cutting propagation technology could enable consistent multiplication of quality plants that are genetically identical to the parent plant. The current study aimed to develop a simple, cost-effective, reproducible, and efficient hydroponic cutting method to preserve alfalfa plants and for molecular research. In this study, alfalfa landrace ‘Wudi’ was grown in hydroponics for 30 days and used as source material for cuttings. The top, middle and bottom sections of its stem were used as cuttings. The rooting rate, root length, and stem height of the different stem sections were compared to determine the best segment for alfalfa propagation in four nutrient treatments (H(M), H(M) + 1/500H, H(M) + 1/1000H and d H(M) + 1/2000H). After 21 days of culture, the rooting rates of all the three stem types under four cutting nutrient solutions were above 78%. The rooting rate of the middle and bottom parts in H(M) + 1/1000 H and H(M) + 1/2000 H nutrient solutions reached more than 93%, with a higher health survey score (> 4.70). In conclusion, this study developed a de novo cutting propagation method that can be used to conserve and propagate germplasm in breeding programs and research. This method is a new report on the cutting propagation of alfalfa by hydroponics, which could supplement the existing cutting propagation methods. Springer India 2021-05-19 2021-06 /pmc/articles/PMC8212189/ /pubmed/34220046 http://dx.doi.org/10.1007/s12298-021-00995-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhao, Zhili
Zhang, Wenyu
Liu, Yang
Li, Shuai
Yao, Wu
Sun, Xiaohui
Li, Siyu
Ma, Lichao
Sun, Juan
Yang, Qingchuan
Li, Yongxiang
Yang, Guofeng
Wang, Zeng-Yu
Cong, Lili
De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
title De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
title_full De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
title_fullStr De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
title_full_unstemmed De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
title_short De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
title_sort de novo hydroponics system efficiency for the cuttings of alfalfa (medicago sativa l.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212189/
https://www.ncbi.nlm.nih.gov/pubmed/34220046
http://dx.doi.org/10.1007/s12298-021-00995-3
work_keys_str_mv AT zhaozhili denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT zhangwenyu denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT liuyang denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT lishuai denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT yaowu denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT sunxiaohui denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT lisiyu denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT malichao denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT sunjuan denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT yangqingchuan denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT liyongxiang denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT yangguofeng denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT wangzengyu denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival
AT conglili denovohydroponicssystemefficiencyforthecuttingsofalfalfamedicagosatival