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De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.)
The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer India
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212189/ https://www.ncbi.nlm.nih.gov/pubmed/34220046 http://dx.doi.org/10.1007/s12298-021-00995-3 |
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author | Zhao, Zhili Zhang, Wenyu Liu, Yang Li, Shuai Yao, Wu Sun, Xiaohui Li, Siyu Ma, Lichao Sun, Juan Yang, Qingchuan Li, Yongxiang Yang, Guofeng Wang, Zeng-Yu Cong, Lili |
author_facet | Zhao, Zhili Zhang, Wenyu Liu, Yang Li, Shuai Yao, Wu Sun, Xiaohui Li, Siyu Ma, Lichao Sun, Juan Yang, Qingchuan Li, Yongxiang Yang, Guofeng Wang, Zeng-Yu Cong, Lili |
author_sort | Zhao, Zhili |
collection | PubMed |
description | The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore, maintaining excellent traits through seed reproduction is a prime challenge in alfalfa. However, the cutting propagation technology could enable consistent multiplication of quality plants that are genetically identical to the parent plant. The current study aimed to develop a simple, cost-effective, reproducible, and efficient hydroponic cutting method to preserve alfalfa plants and for molecular research. In this study, alfalfa landrace ‘Wudi’ was grown in hydroponics for 30 days and used as source material for cuttings. The top, middle and bottom sections of its stem were used as cuttings. The rooting rate, root length, and stem height of the different stem sections were compared to determine the best segment for alfalfa propagation in four nutrient treatments (H(M), H(M) + 1/500H, H(M) + 1/1000H and d H(M) + 1/2000H). After 21 days of culture, the rooting rates of all the three stem types under four cutting nutrient solutions were above 78%. The rooting rate of the middle and bottom parts in H(M) + 1/1000 H and H(M) + 1/2000 H nutrient solutions reached more than 93%, with a higher health survey score (> 4.70). In conclusion, this study developed a de novo cutting propagation method that can be used to conserve and propagate germplasm in breeding programs and research. This method is a new report on the cutting propagation of alfalfa by hydroponics, which could supplement the existing cutting propagation methods. |
format | Online Article Text |
id | pubmed-8212189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer India |
record_format | MEDLINE/PubMed |
spelling | pubmed-82121892021-07-01 De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) Zhao, Zhili Zhang, Wenyu Liu, Yang Li, Shuai Yao, Wu Sun, Xiaohui Li, Siyu Ma, Lichao Sun, Juan Yang, Qingchuan Li, Yongxiang Yang, Guofeng Wang, Zeng-Yu Cong, Lili Physiol Mol Biol Plants Research Article The legume plant alfalfa (Medicago sativa L.) is a widely cultivated perennial forage due to its high protein content, palatability, and strong adaptability to diverse agro-ecological zones. Alfalfa is a self-incompatible cross-pollinated autotetraploid species with tetrasomic inheritance. Therefore, maintaining excellent traits through seed reproduction is a prime challenge in alfalfa. However, the cutting propagation technology could enable consistent multiplication of quality plants that are genetically identical to the parent plant. The current study aimed to develop a simple, cost-effective, reproducible, and efficient hydroponic cutting method to preserve alfalfa plants and for molecular research. In this study, alfalfa landrace ‘Wudi’ was grown in hydroponics for 30 days and used as source material for cuttings. The top, middle and bottom sections of its stem were used as cuttings. The rooting rate, root length, and stem height of the different stem sections were compared to determine the best segment for alfalfa propagation in four nutrient treatments (H(M), H(M) + 1/500H, H(M) + 1/1000H and d H(M) + 1/2000H). After 21 days of culture, the rooting rates of all the three stem types under four cutting nutrient solutions were above 78%. The rooting rate of the middle and bottom parts in H(M) + 1/1000 H and H(M) + 1/2000 H nutrient solutions reached more than 93%, with a higher health survey score (> 4.70). In conclusion, this study developed a de novo cutting propagation method that can be used to conserve and propagate germplasm in breeding programs and research. This method is a new report on the cutting propagation of alfalfa by hydroponics, which could supplement the existing cutting propagation methods. Springer India 2021-05-19 2021-06 /pmc/articles/PMC8212189/ /pubmed/34220046 http://dx.doi.org/10.1007/s12298-021-00995-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Zhao, Zhili Zhang, Wenyu Liu, Yang Li, Shuai Yao, Wu Sun, Xiaohui Li, Siyu Ma, Lichao Sun, Juan Yang, Qingchuan Li, Yongxiang Yang, Guofeng Wang, Zeng-Yu Cong, Lili De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) |
title | De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) |
title_full | De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) |
title_fullStr | De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) |
title_full_unstemmed | De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) |
title_short | De novo hydroponics system efficiency for the cuttings of alfalfa (Medicago sativa L.) |
title_sort | de novo hydroponics system efficiency for the cuttings of alfalfa (medicago sativa l.) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212189/ https://www.ncbi.nlm.nih.gov/pubmed/34220046 http://dx.doi.org/10.1007/s12298-021-00995-3 |
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