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Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells

BACKGROUND: A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-spe...

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Autores principales: Chankeaw, Wiruntita, Lignier, Sandra, Richard, Christophe, Ntallaris, Theodoros, Raliou, Mariam, Guo, Yongzhi, Plassard, Damien, Bevilacqua, Claudia, Sandra, Olivier, Andersson, Göran, Humblot, Patrice, Charpigny, Gilles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212485/
https://www.ncbi.nlm.nih.gov/pubmed/34139994
http://dx.doi.org/10.1186/s12864-021-07712-0
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author Chankeaw, Wiruntita
Lignier, Sandra
Richard, Christophe
Ntallaris, Theodoros
Raliou, Mariam
Guo, Yongzhi
Plassard, Damien
Bevilacqua, Claudia
Sandra, Olivier
Andersson, Göran
Humblot, Patrice
Charpigny, Gilles
author_facet Chankeaw, Wiruntita
Lignier, Sandra
Richard, Christophe
Ntallaris, Theodoros
Raliou, Mariam
Guo, Yongzhi
Plassard, Damien
Bevilacqua, Claudia
Sandra, Olivier
Andersson, Göran
Humblot, Patrice
Charpigny, Gilles
author_sort Chankeaw, Wiruntita
collection PubMed
description BACKGROUND: A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-specific gene expression, when analysis performed with whole endometrium, is still weak and obviously limits the relevance of the results of gene expression studies. Thus, the aim of this study was to characterize specific transcriptome of the three main cell-types of the bovine endometrium at day-15 of the estrus cycle. RESULTS: In the RNA-Seq analysis, the number of expressed genes detected over 10 transcripts per million was 6622, 7814 and 8242 for LE, GE and ST respectively. ST expressed exclusively 1236 genes while only 551 transcripts were specific to the GE and 330 specific to LE. For ST, over-represented biological processes included many regulation processes and response to stimulus, cell communication and cell adhesion, extracellular matrix organization as well as developmental process. For GE, cilium organization, cilium movement, protein localization to cilium and microtubule-based process were the only four main biological processes enriched. For LE, over-represented biological processes were enzyme linked receptor protein signaling pathway, cell-substrate adhesion and circulatory system process. CONCLUSION: The data show that each endometrial cell-type has a distinct molecular signature and provide a significantly improved overview on the biological process supported by specific cell-types. The most interesting result is that stromal cells express more genes than the two epithelial types and are associated with a greater number of pathways and ontology terms. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07712-0.
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spelling pubmed-82124852021-06-22 Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells Chankeaw, Wiruntita Lignier, Sandra Richard, Christophe Ntallaris, Theodoros Raliou, Mariam Guo, Yongzhi Plassard, Damien Bevilacqua, Claudia Sandra, Olivier Andersson, Göran Humblot, Patrice Charpigny, Gilles BMC Genomics Research Article BACKGROUND: A number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of pregnancy. However, to date, these studies have been performed on the whole endometrium which is a complex tissue. Consequently, the knowledge of cell-specific gene expression, when analysis performed with whole endometrium, is still weak and obviously limits the relevance of the results of gene expression studies. Thus, the aim of this study was to characterize specific transcriptome of the three main cell-types of the bovine endometrium at day-15 of the estrus cycle. RESULTS: In the RNA-Seq analysis, the number of expressed genes detected over 10 transcripts per million was 6622, 7814 and 8242 for LE, GE and ST respectively. ST expressed exclusively 1236 genes while only 551 transcripts were specific to the GE and 330 specific to LE. For ST, over-represented biological processes included many regulation processes and response to stimulus, cell communication and cell adhesion, extracellular matrix organization as well as developmental process. For GE, cilium organization, cilium movement, protein localization to cilium and microtubule-based process were the only four main biological processes enriched. For LE, over-represented biological processes were enzyme linked receptor protein signaling pathway, cell-substrate adhesion and circulatory system process. CONCLUSION: The data show that each endometrial cell-type has a distinct molecular signature and provide a significantly improved overview on the biological process supported by specific cell-types. The most interesting result is that stromal cells express more genes than the two epithelial types and are associated with a greater number of pathways and ontology terms. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07712-0. BioMed Central 2021-06-18 /pmc/articles/PMC8212485/ /pubmed/34139994 http://dx.doi.org/10.1186/s12864-021-07712-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Chankeaw, Wiruntita
Lignier, Sandra
Richard, Christophe
Ntallaris, Theodoros
Raliou, Mariam
Guo, Yongzhi
Plassard, Damien
Bevilacqua, Claudia
Sandra, Olivier
Andersson, Göran
Humblot, Patrice
Charpigny, Gilles
Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
title Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
title_full Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
title_fullStr Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
title_full_unstemmed Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
title_short Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
title_sort analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212485/
https://www.ncbi.nlm.nih.gov/pubmed/34139994
http://dx.doi.org/10.1186/s12864-021-07712-0
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