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Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances

It is well known that WRKY transcription factors play essential roles in plants’ response to diverse stress responses, especially to drought and salt stresses. However, a full comprehensive analysis of this family in wheat is still missing. Here we used in silico analysis and identified 124 WRKY gen...

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Autores principales: Ye, Hong, Qiao, Linyi, Guo, Haoyu, Guo, Liping, Ren, Fei, Bai, Jianfang, Wang, Yukun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212938/
https://www.ncbi.nlm.nih.gov/pubmed/34149760
http://dx.doi.org/10.3389/fpls.2021.663118
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author Ye, Hong
Qiao, Linyi
Guo, Haoyu
Guo, Liping
Ren, Fei
Bai, Jianfang
Wang, Yukun
author_facet Ye, Hong
Qiao, Linyi
Guo, Haoyu
Guo, Liping
Ren, Fei
Bai, Jianfang
Wang, Yukun
author_sort Ye, Hong
collection PubMed
description It is well known that WRKY transcription factors play essential roles in plants’ response to diverse stress responses, especially to drought and salt stresses. However, a full comprehensive analysis of this family in wheat is still missing. Here we used in silico analysis and identified 124 WRKY genes, including 294 homeologous copies from a high-quality reference genome of wheat (Triticum aestivum). We also found that the TaWRKY gene family did not undergo gene duplication rather than gene loss during the evolutionary process. The TaWRKY family members displayed different expression profiles under several abiotic stresses, indicating their unique functions in the mediation of particular responses. Furthermore, TaWRKY75-A was highly induced after polyethylene glycol and salt treatments. The ectopic expression of TaWRKY75-A in Arabidopsis enhanced drought and salt tolerance. A comparative transcriptome analysis demonstrated that TaWRKY75-A integrated jasmonic acid biosynthetic pathway and other potential metabolic pathways to increase drought and salt resistances in transgenic Arabidopsis. Our study provides valuable insights into the WRKY family in wheat and will generate a useful genetic resource for improving wheat breeding.
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spelling pubmed-82129382021-06-19 Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances Ye, Hong Qiao, Linyi Guo, Haoyu Guo, Liping Ren, Fei Bai, Jianfang Wang, Yukun Front Plant Sci Plant Science It is well known that WRKY transcription factors play essential roles in plants’ response to diverse stress responses, especially to drought and salt stresses. However, a full comprehensive analysis of this family in wheat is still missing. Here we used in silico analysis and identified 124 WRKY genes, including 294 homeologous copies from a high-quality reference genome of wheat (Triticum aestivum). We also found that the TaWRKY gene family did not undergo gene duplication rather than gene loss during the evolutionary process. The TaWRKY family members displayed different expression profiles under several abiotic stresses, indicating their unique functions in the mediation of particular responses. Furthermore, TaWRKY75-A was highly induced after polyethylene glycol and salt treatments. The ectopic expression of TaWRKY75-A in Arabidopsis enhanced drought and salt tolerance. A comparative transcriptome analysis demonstrated that TaWRKY75-A integrated jasmonic acid biosynthetic pathway and other potential metabolic pathways to increase drought and salt resistances in transgenic Arabidopsis. Our study provides valuable insights into the WRKY family in wheat and will generate a useful genetic resource for improving wheat breeding. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212938/ /pubmed/34149760 http://dx.doi.org/10.3389/fpls.2021.663118 Text en Copyright © 2021 Ye, Qiao, Guo, Guo, Ren, Bai and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ye, Hong
Qiao, Linyi
Guo, Haoyu
Guo, Liping
Ren, Fei
Bai, Jianfang
Wang, Yukun
Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
title Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
title_full Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
title_fullStr Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
title_full_unstemmed Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
title_short Genome-Wide Identification of Wheat WRKY Gene Family Reveals That TaWRKY75-A Is Referred to Drought and Salt Resistances
title_sort genome-wide identification of wheat wrky gene family reveals that tawrky75-a is referred to drought and salt resistances
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212938/
https://www.ncbi.nlm.nih.gov/pubmed/34149760
http://dx.doi.org/10.3389/fpls.2021.663118
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