Cargando…
Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus
The acquisition of the phage-encoded superantigen ssa by scarlet fever-associated group A Streptococcus (Streptococcus pyogenes, GAS) is found in North Asia. Nonetheless, the impact of acquiring ssa by GAS in invasive infections is unclear. This study initially analyzed the prevalence of ssa+ GAS am...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212969/ https://www.ncbi.nlm.nih.gov/pubmed/34149675 http://dx.doi.org/10.3389/fmicb.2021.685343 |
_version_ | 1783709741316308992 |
---|---|
author | Chiang-Ni, Chuan Liu, Yen-Shan Lin, Chieh-Yu Hsu, Chih-Yun Shi, Yong-An Chen, Yi-Ywan M. Lai, Chih-Ho Chiu, Cheng-Hsun |
author_facet | Chiang-Ni, Chuan Liu, Yen-Shan Lin, Chieh-Yu Hsu, Chih-Yun Shi, Yong-An Chen, Yi-Ywan M. Lai, Chih-Ho Chiu, Cheng-Hsun |
author_sort | Chiang-Ni, Chuan |
collection | PubMed |
description | The acquisition of the phage-encoded superantigen ssa by scarlet fever-associated group A Streptococcus (Streptococcus pyogenes, GAS) is found in North Asia. Nonetheless, the impact of acquiring ssa by GAS in invasive infections is unclear. This study initially analyzed the prevalence of ssa+ GAS among isolates from sterile tissues and blood. Among 220 isolates in northern Taiwan, the prevalence of ssa+ isolates increased from 1.5% in 2008–2010 to 40% in 2017–2019. Spontaneous mutations in covR/covS, which result in the functional loss of capacity to phosphorylate CovR, are frequently recovered from GAS invasive infection cases. Consistent with this, Phostag western blot results indicated that among the invasive infection isolates studied, 10% of the ssa+ isolates lacked detectable phosphorylated CovR. Transcription of ssa is upregulated in the covS mutant. Furthermore, in emm1 and emm12 covS mutants, ssa deletion significantly reduced their capacity to grow in human whole blood. Finally, this study showed that the ssa gene could be transferred from emm12-type isolates to the emm1-type wild-type strain and covS mutants through phage infection and lysogenic conversion. As the prevalence of ssa+ isolates increased significantly, the role of streptococcal superantigen in GAS pathogenesis, particularly in invasive covR/covS mutants, should be further analyzed. |
format | Online Article Text |
id | pubmed-8212969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82129692021-06-19 Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus Chiang-Ni, Chuan Liu, Yen-Shan Lin, Chieh-Yu Hsu, Chih-Yun Shi, Yong-An Chen, Yi-Ywan M. Lai, Chih-Ho Chiu, Cheng-Hsun Front Microbiol Microbiology The acquisition of the phage-encoded superantigen ssa by scarlet fever-associated group A Streptococcus (Streptococcus pyogenes, GAS) is found in North Asia. Nonetheless, the impact of acquiring ssa by GAS in invasive infections is unclear. This study initially analyzed the prevalence of ssa+ GAS among isolates from sterile tissues and blood. Among 220 isolates in northern Taiwan, the prevalence of ssa+ isolates increased from 1.5% in 2008–2010 to 40% in 2017–2019. Spontaneous mutations in covR/covS, which result in the functional loss of capacity to phosphorylate CovR, are frequently recovered from GAS invasive infection cases. Consistent with this, Phostag western blot results indicated that among the invasive infection isolates studied, 10% of the ssa+ isolates lacked detectable phosphorylated CovR. Transcription of ssa is upregulated in the covS mutant. Furthermore, in emm1 and emm12 covS mutants, ssa deletion significantly reduced their capacity to grow in human whole blood. Finally, this study showed that the ssa gene could be transferred from emm12-type isolates to the emm1-type wild-type strain and covS mutants through phage infection and lysogenic conversion. As the prevalence of ssa+ isolates increased significantly, the role of streptococcal superantigen in GAS pathogenesis, particularly in invasive covR/covS mutants, should be further analyzed. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212969/ /pubmed/34149675 http://dx.doi.org/10.3389/fmicb.2021.685343 Text en Copyright © 2021 Chiang-Ni, Liu, Lin, Hsu, Shi, Chen, Lai and Chiu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chiang-Ni, Chuan Liu, Yen-Shan Lin, Chieh-Yu Hsu, Chih-Yun Shi, Yong-An Chen, Yi-Ywan M. Lai, Chih-Ho Chiu, Cheng-Hsun Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus |
title | Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus
|
title_full | Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus
|
title_fullStr | Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus
|
title_full_unstemmed | Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus
|
title_short | Incidence and Effects of Acquisition of the Phage-Encoded ssa Superantigen Gene in Invasive Group A Streptococcus
|
title_sort | incidence and effects of acquisition of the phage-encoded ssa superantigen gene in invasive group a streptococcus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212969/ https://www.ncbi.nlm.nih.gov/pubmed/34149675 http://dx.doi.org/10.3389/fmicb.2021.685343 |
work_keys_str_mv | AT chiangnichuan incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT liuyenshan incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT linchiehyu incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT hsuchihyun incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT shiyongan incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT chenyiywanm incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT laichihho incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus AT chiuchenghsun incidenceandeffectsofacquisitionofthephageencodedssasuperantigengeneininvasivegroupastreptococcus |