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Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue mode...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212980/ https://www.ncbi.nlm.nih.gov/pubmed/34150901 http://dx.doi.org/10.3389/fvets.2021.686088 |
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author | Majorova, Dominika Atkins, Elizabeth Martineau, Henny Vokral, Ivan Oosterhuis, Dorenda Olinga, Peter Wren, Brendan Cuccui, Jon Werling, Dirk |
author_facet | Majorova, Dominika Atkins, Elizabeth Martineau, Henny Vokral, Ivan Oosterhuis, Dorenda Olinga, Peter Wren, Brendan Cuccui, Jon Werling, Dirk |
author_sort | Majorova, Dominika |
collection | PubMed |
description | The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches. |
format | Online Article Text |
id | pubmed-8212980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82129802021-06-19 Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction Majorova, Dominika Atkins, Elizabeth Martineau, Henny Vokral, Ivan Oosterhuis, Dorenda Olinga, Peter Wren, Brendan Cuccui, Jon Werling, Dirk Front Vet Sci Veterinary Science The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212980/ /pubmed/34150901 http://dx.doi.org/10.3389/fvets.2021.686088 Text en Copyright © 2021 Majorova, Atkins, Martineau, Vokral, Oosterhuis, Olinga, Wren, Cuccui and Werling. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Majorova, Dominika Atkins, Elizabeth Martineau, Henny Vokral, Ivan Oosterhuis, Dorenda Olinga, Peter Wren, Brendan Cuccui, Jon Werling, Dirk Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction |
title | Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction |
title_full | Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction |
title_fullStr | Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction |
title_full_unstemmed | Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction |
title_short | Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction |
title_sort | use of precision-cut tissue slices as a translational model to study host-pathogen interaction |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212980/ https://www.ncbi.nlm.nih.gov/pubmed/34150901 http://dx.doi.org/10.3389/fvets.2021.686088 |
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