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Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction

The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue mode...

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Autores principales: Majorova, Dominika, Atkins, Elizabeth, Martineau, Henny, Vokral, Ivan, Oosterhuis, Dorenda, Olinga, Peter, Wren, Brendan, Cuccui, Jon, Werling, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212980/
https://www.ncbi.nlm.nih.gov/pubmed/34150901
http://dx.doi.org/10.3389/fvets.2021.686088
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author Majorova, Dominika
Atkins, Elizabeth
Martineau, Henny
Vokral, Ivan
Oosterhuis, Dorenda
Olinga, Peter
Wren, Brendan
Cuccui, Jon
Werling, Dirk
author_facet Majorova, Dominika
Atkins, Elizabeth
Martineau, Henny
Vokral, Ivan
Oosterhuis, Dorenda
Olinga, Peter
Wren, Brendan
Cuccui, Jon
Werling, Dirk
author_sort Majorova, Dominika
collection PubMed
description The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches.
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spelling pubmed-82129802021-06-19 Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction Majorova, Dominika Atkins, Elizabeth Martineau, Henny Vokral, Ivan Oosterhuis, Dorenda Olinga, Peter Wren, Brendan Cuccui, Jon Werling, Dirk Front Vet Sci Veterinary Science The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8212980/ /pubmed/34150901 http://dx.doi.org/10.3389/fvets.2021.686088 Text en Copyright © 2021 Majorova, Atkins, Martineau, Vokral, Oosterhuis, Olinga, Wren, Cuccui and Werling. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Majorova, Dominika
Atkins, Elizabeth
Martineau, Henny
Vokral, Ivan
Oosterhuis, Dorenda
Olinga, Peter
Wren, Brendan
Cuccui, Jon
Werling, Dirk
Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
title Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
title_full Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
title_fullStr Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
title_full_unstemmed Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
title_short Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction
title_sort use of precision-cut tissue slices as a translational model to study host-pathogen interaction
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212980/
https://www.ncbi.nlm.nih.gov/pubmed/34150901
http://dx.doi.org/10.3389/fvets.2021.686088
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