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Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing
The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and prolifer...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213172/ https://www.ncbi.nlm.nih.gov/pubmed/34143844 http://dx.doi.org/10.1371/journal.pone.0253669 |
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author | Alishahedani, Mohammadali E. Yadav, Manoj McCann, Katelyn J. Gough, Portia Castillo, Carlos R. Matriz, Jobel Myles, Ian A. |
author_facet | Alishahedani, Mohammadali E. Yadav, Manoj McCann, Katelyn J. Gough, Portia Castillo, Carlos R. Matriz, Jobel Myles, Ian A. |
author_sort | Alishahedani, Mohammadali E. |
collection | PubMed |
description | The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to ‘heal’ the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted. |
format | Online Article Text |
id | pubmed-8213172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82131722021-06-29 Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing Alishahedani, Mohammadali E. Yadav, Manoj McCann, Katelyn J. Gough, Portia Castillo, Carlos R. Matriz, Jobel Myles, Ian A. PLoS One Research Article The scratch assay is an in vitro technique used to analyze cell migration, proliferation, and cell-to-cell interaction. In the assay, cells are grown to confluence and then ‘scratched’ with a sterile instrument. For the cells in the leading edge, the resulting polarity induces migration and proliferation in attempt to ‘heal’ the modeled wound. Keloid scars are known to have an accelerated wound closure phenotype in the scratch assay, representing an overactivation of wound healing. We performed a qualitative review of the recent literature searching for inhibitors of scratch assay activity that were already available in topical formulations under the hypothesis that such compounds may offer therapeutic potential in keloid treatment. Although several shortcomings in the scratch assay literature were identified, caffeine and allicin successfully inhibited the scratch assay closure and inflammatory abnormalities in the commercially available keloid fibroblast cell line. Caffeine and allicin also impacted ATP production in keloid cells, most notably with inhibition of non-mitochondrial oxygen consumption. The traditional Chinese medicine, shikonin, was also successful in inhibiting scratch closure but displayed less dramatic impacts on metabolism. Together, our results partially summarize the strengths and limitations of current scratch assay literature and suggest clinical assessment of the therapeutic potential for these identified compounds against keloid scars may be warranted. Public Library of Science 2021-06-18 /pmc/articles/PMC8213172/ /pubmed/34143844 http://dx.doi.org/10.1371/journal.pone.0253669 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Alishahedani, Mohammadali E. Yadav, Manoj McCann, Katelyn J. Gough, Portia Castillo, Carlos R. Matriz, Jobel Myles, Ian A. Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
title | Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
title_full | Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
title_fullStr | Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
title_full_unstemmed | Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
title_short | Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
title_sort | therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213172/ https://www.ncbi.nlm.nih.gov/pubmed/34143844 http://dx.doi.org/10.1371/journal.pone.0253669 |
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