Cargando…

Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt

Staphylococci can cause a wide array of infections that can be life threatening. These infections become more deadly when the isolates are antibiotic resistant and thus harder to treat. Many resistance determinants are plasmid-mediated; however, staphylococcal plasmids have not yet been fully charac...

Descripción completa

Detalles Bibliográficos
Autores principales: Mores, Carine R., Montelongo, Cesar, Putonti, Catherine, Wolfe, Alan J., Abouelfetouh, Alaa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213342/
https://www.ncbi.nlm.nih.gov/pubmed/34149648
http://dx.doi.org/10.3389/fmicb.2021.659116
_version_ 1783709826020278272
author Mores, Carine R.
Montelongo, Cesar
Putonti, Catherine
Wolfe, Alan J.
Abouelfetouh, Alaa
author_facet Mores, Carine R.
Montelongo, Cesar
Putonti, Catherine
Wolfe, Alan J.
Abouelfetouh, Alaa
author_sort Mores, Carine R.
collection PubMed
description Staphylococci can cause a wide array of infections that can be life threatening. These infections become more deadly when the isolates are antibiotic resistant and thus harder to treat. Many resistance determinants are plasmid-mediated; however, staphylococcal plasmids have not yet been fully characterized. In particular, plasmids and their contributions to antibiotic resistance have not been investigated within the Arab states, where antibiotic use is not universally regulated. Here, we characterized the putative plasmid content among 56 Staphylococcus aureus and 10 Staphylococcus haemolyticus clinical isolates from Alexandria, Egypt. Putative plasmid sequences were detected in over half of our collection. In total, we identified 72 putative plasmid sequences in 27 S. aureus and 1 S. haemolyticus isolates. While these isolates typically carried one or two plasmids, we identified one isolate—S. aureus AA53—with 11 putative plasmids. The plasmid sequences most frequently encoded a Rep_1, RepL, or PriCT_1 type replication protein. As expected, antibiotic resistance genes were widespread among the identified plasmid sequences. Related plasmids were identified amongst our clinical isolates; homologous plasmids present in multiple isolates clustered into 11 groups based upon sequence similarity. Plasmids from the same cluster often shared antibiotic resistance genes, including blaZ, which is associated with β-lactam resistance. Our analyses suggest that plasmids are a key factor in the pathology and epidemiology of S. aureus in Egypt. A better characterization of plasmids and the role they contribute to the success of Staphylococci as pathogens will guide the design of effective control strategies to limit their spread.
format Online
Article
Text
id pubmed-8213342
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82133422021-06-19 Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt Mores, Carine R. Montelongo, Cesar Putonti, Catherine Wolfe, Alan J. Abouelfetouh, Alaa Front Microbiol Microbiology Staphylococci can cause a wide array of infections that can be life threatening. These infections become more deadly when the isolates are antibiotic resistant and thus harder to treat. Many resistance determinants are plasmid-mediated; however, staphylococcal plasmids have not yet been fully characterized. In particular, plasmids and their contributions to antibiotic resistance have not been investigated within the Arab states, where antibiotic use is not universally regulated. Here, we characterized the putative plasmid content among 56 Staphylococcus aureus and 10 Staphylococcus haemolyticus clinical isolates from Alexandria, Egypt. Putative plasmid sequences were detected in over half of our collection. In total, we identified 72 putative plasmid sequences in 27 S. aureus and 1 S. haemolyticus isolates. While these isolates typically carried one or two plasmids, we identified one isolate—S. aureus AA53—with 11 putative plasmids. The plasmid sequences most frequently encoded a Rep_1, RepL, or PriCT_1 type replication protein. As expected, antibiotic resistance genes were widespread among the identified plasmid sequences. Related plasmids were identified amongst our clinical isolates; homologous plasmids present in multiple isolates clustered into 11 groups based upon sequence similarity. Plasmids from the same cluster often shared antibiotic resistance genes, including blaZ, which is associated with β-lactam resistance. Our analyses suggest that plasmids are a key factor in the pathology and epidemiology of S. aureus in Egypt. A better characterization of plasmids and the role they contribute to the success of Staphylococci as pathogens will guide the design of effective control strategies to limit their spread. Frontiers Media S.A. 2021-06-04 /pmc/articles/PMC8213342/ /pubmed/34149648 http://dx.doi.org/10.3389/fmicb.2021.659116 Text en Copyright © 2021 Mores, Montelongo, Putonti, Wolfe and Abouelfetouh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mores, Carine R.
Montelongo, Cesar
Putonti, Catherine
Wolfe, Alan J.
Abouelfetouh, Alaa
Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt
title Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt
title_full Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt
title_fullStr Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt
title_full_unstemmed Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt
title_short Investigation of Plasmids Among Clinical Staphylococcus aureus and Staphylococcus haemolyticus Isolates From Egypt
title_sort investigation of plasmids among clinical staphylococcus aureus and staphylococcus haemolyticus isolates from egypt
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213342/
https://www.ncbi.nlm.nih.gov/pubmed/34149648
http://dx.doi.org/10.3389/fmicb.2021.659116
work_keys_str_mv AT morescariner investigationofplasmidsamongclinicalstaphylococcusaureusandstaphylococcushaemolyticusisolatesfromegypt
AT montelongocesar investigationofplasmidsamongclinicalstaphylococcusaureusandstaphylococcushaemolyticusisolatesfromegypt
AT putonticatherine investigationofplasmidsamongclinicalstaphylococcusaureusandstaphylococcushaemolyticusisolatesfromegypt
AT wolfealanj investigationofplasmidsamongclinicalstaphylococcusaureusandstaphylococcushaemolyticusisolatesfromegypt
AT abouelfetouhalaa investigationofplasmidsamongclinicalstaphylococcusaureusandstaphylococcushaemolyticusisolatesfromegypt