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OsJAZ11 regulates phosphate starvation responses in rice
MAIN CONCLUSION: OsJAZ11 regulates phosphate homeostasis by suppressing jasmonic acid signaling and biosynthesis in rice roots. ABSTRACT: Jasmonic Acid (JA) is a key plant signaling molecule which negatively regulates growth processes including root elongation. JAZ (JASMONATE ZIM-DOMAIN) proteins fu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213676/ https://www.ncbi.nlm.nih.gov/pubmed/34143292 http://dx.doi.org/10.1007/s00425-021-03657-6 |
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author | Pandey, Bipin K. Verma, Lokesh Prusty, Ankita Singh, Ajit Pal Bennett, Malcolm J. Tyagi, Akhilesh K. Giri, Jitender Mehra, Poonam |
author_facet | Pandey, Bipin K. Verma, Lokesh Prusty, Ankita Singh, Ajit Pal Bennett, Malcolm J. Tyagi, Akhilesh K. Giri, Jitender Mehra, Poonam |
author_sort | Pandey, Bipin K. |
collection | PubMed |
description | MAIN CONCLUSION: OsJAZ11 regulates phosphate homeostasis by suppressing jasmonic acid signaling and biosynthesis in rice roots. ABSTRACT: Jasmonic Acid (JA) is a key plant signaling molecule which negatively regulates growth processes including root elongation. JAZ (JASMONATE ZIM-DOMAIN) proteins function as transcriptional repressors of JA signaling. Therefore, targeting JA signaling by deploying JAZ repressors may enhance root length in crops. In this study, we overexpressed JAZ repressor OsJAZ11 in rice to alleviate the root growth inhibitory action of JA. OsJAZ11 is a low phosphate (Pi) responsive gene which is transcriptionally regulated by OsPHR2. We report that OsJAZ11 overexpression promoted primary and seminal root elongation which enhanced Pi foraging. Expression studies revealed that overexpression of OsJAZ11 also reduced Pi starvation response (PSR) under Pi limiting conditions. Moreover, OsJAZ11 overexpression also suppressed JA signaling and biosynthesis as compared to wild type (WT). We further demonstrated that the C-terminal region of OsJAZ11 was crucial for stimulating root elongation in overexpression lines. Rice transgenics overexpressing truncated OsJAZ11ΔC transgene (i.e., missing C-terminal region) exhibited reduced root length and Pi uptake. Interestingly, OsJAZ11 also regulates Pi homeostasis via physical interaction with a key Pi sensing protein, OsSPX1. Our study highlights the functional connections between JA and Pi signaling and reveals JAZ repressors as a promising candidate for improving low Pi tolerance of elite rice genotypes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00425-021-03657-6. |
format | Online Article Text |
id | pubmed-8213676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-82136762021-07-01 OsJAZ11 regulates phosphate starvation responses in rice Pandey, Bipin K. Verma, Lokesh Prusty, Ankita Singh, Ajit Pal Bennett, Malcolm J. Tyagi, Akhilesh K. Giri, Jitender Mehra, Poonam Planta Original Article MAIN CONCLUSION: OsJAZ11 regulates phosphate homeostasis by suppressing jasmonic acid signaling and biosynthesis in rice roots. ABSTRACT: Jasmonic Acid (JA) is a key plant signaling molecule which negatively regulates growth processes including root elongation. JAZ (JASMONATE ZIM-DOMAIN) proteins function as transcriptional repressors of JA signaling. Therefore, targeting JA signaling by deploying JAZ repressors may enhance root length in crops. In this study, we overexpressed JAZ repressor OsJAZ11 in rice to alleviate the root growth inhibitory action of JA. OsJAZ11 is a low phosphate (Pi) responsive gene which is transcriptionally regulated by OsPHR2. We report that OsJAZ11 overexpression promoted primary and seminal root elongation which enhanced Pi foraging. Expression studies revealed that overexpression of OsJAZ11 also reduced Pi starvation response (PSR) under Pi limiting conditions. Moreover, OsJAZ11 overexpression also suppressed JA signaling and biosynthesis as compared to wild type (WT). We further demonstrated that the C-terminal region of OsJAZ11 was crucial for stimulating root elongation in overexpression lines. Rice transgenics overexpressing truncated OsJAZ11ΔC transgene (i.e., missing C-terminal region) exhibited reduced root length and Pi uptake. Interestingly, OsJAZ11 also regulates Pi homeostasis via physical interaction with a key Pi sensing protein, OsSPX1. Our study highlights the functional connections between JA and Pi signaling and reveals JAZ repressors as a promising candidate for improving low Pi tolerance of elite rice genotypes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00425-021-03657-6. Springer Berlin Heidelberg 2021-06-18 2021 /pmc/articles/PMC8213676/ /pubmed/34143292 http://dx.doi.org/10.1007/s00425-021-03657-6 Text en © Crown 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Pandey, Bipin K. Verma, Lokesh Prusty, Ankita Singh, Ajit Pal Bennett, Malcolm J. Tyagi, Akhilesh K. Giri, Jitender Mehra, Poonam OsJAZ11 regulates phosphate starvation responses in rice |
title | OsJAZ11 regulates phosphate starvation responses in rice |
title_full | OsJAZ11 regulates phosphate starvation responses in rice |
title_fullStr | OsJAZ11 regulates phosphate starvation responses in rice |
title_full_unstemmed | OsJAZ11 regulates phosphate starvation responses in rice |
title_short | OsJAZ11 regulates phosphate starvation responses in rice |
title_sort | osjaz11 regulates phosphate starvation responses in rice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213676/ https://www.ncbi.nlm.nih.gov/pubmed/34143292 http://dx.doi.org/10.1007/s00425-021-03657-6 |
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