Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells

The cell biology of circadian clocks is still in its infancy. Here, we describe an efficient strategy for generating knock-in reporter cell lines using CRISPR technology that is particularly useful for genes expressed transiently or at low levels, such as those coding for circadian clock proteins. W...

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Detalles Bibliográficos
Autores principales: Gabriel, Christian H., del Olmo, Marta, Zehtabian, Amin, Jäger, Marten, Reischl, Silke, van Dijk, Hannah, Ulbricht, Carolin, Rakhymzhan, Asylkhan, Korte, Thomas, Koller, Barbara, Grudziecki, Astrid, Maier, Bert, Herrmann, Andreas, Niesner, Raluca, Zemojtel, Tomasz, Ewers, Helge, Granada, Adrián E., Herzel, Hanspeter, Kramer, Achim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8213786/
https://www.ncbi.nlm.nih.gov/pubmed/34145278
http://dx.doi.org/10.1038/s41467-021-24086-9

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