A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients

One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-...

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Autores principales: Goh, Yun Shan, Ng, Lisa F.P., Renia, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214198/
https://www.ncbi.nlm.nih.gov/pubmed/34179835
http://dx.doi.org/10.1016/j.xpro.2021.100671
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author Goh, Yun Shan
Ng, Lisa F.P.
Renia, Laurent
author_facet Goh, Yun Shan
Ng, Lisa F.P.
Renia, Laurent
author_sort Goh, Yun Shan
collection PubMed
description One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021).
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spelling pubmed-82141982021-06-21 A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients Goh, Yun Shan Ng, Lisa F.P. Renia, Laurent STAR Protoc Protocol One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021). Elsevier 2021-06-19 /pmc/articles/PMC8214198/ /pubmed/34179835 http://dx.doi.org/10.1016/j.xpro.2021.100671 Text en © 2021 Agency for Science, Technology and Research https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Goh, Yun Shan
Ng, Lisa F.P.
Renia, Laurent
A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
title A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
title_full A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
title_fullStr A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
title_full_unstemmed A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
title_short A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients
title_sort flow cytometry-based assay for serological detection of anti-spike antibodies in covid-19 patients
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214198/
https://www.ncbi.nlm.nih.gov/pubmed/34179835
http://dx.doi.org/10.1016/j.xpro.2021.100671
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