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Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis

Actinic prurigo (AP) is a type of photodermatosis that primarily affects the Latin American mestizo population. Histologically, AP cheilitis exhibits acanthosis with spongiosis and vacuolation of the basal cell layer overlying a dense lymphocytic inflammatory infiltrate that forms well-defined lymph...

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Autores principales: Cedeño-Suárez, Lily Margoth, Vega-Memije, María Elisa, Cuevas-González, Juan Carlos, Mosqueda-Taylor, Adalberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital Universitário da Universidade de São Paulo 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214903/
https://www.ncbi.nlm.nih.gov/pubmed/34307214
http://dx.doi.org/10.4322/acr.2021.247
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author Cedeño-Suárez, Lily Margoth
Vega-Memije, María Elisa
Cuevas-González, Juan Carlos
Mosqueda-Taylor, Adalberto
author_facet Cedeño-Suárez, Lily Margoth
Vega-Memije, María Elisa
Cuevas-González, Juan Carlos
Mosqueda-Taylor, Adalberto
author_sort Cedeño-Suárez, Lily Margoth
collection PubMed
description Actinic prurigo (AP) is a type of photodermatosis that primarily affects the Latin American mestizo population. Histologically, AP cheilitis exhibits acanthosis with spongiosis and vacuolation of the basal cell layer overlying a dense lymphocytic inflammatory infiltrate that forms well-defined lymphoid follicles. Toluidine blue is a thiazide, acidophilic, and metachromatic dye used in vivo to selectively stain the acidic components of tissues such as sulfates, carboxylates, and phosphate radicals that are incorporated into DNA and RNA. It is necessary to develop a method that allows detecting, on clinical grounds the area of the lesion in which it is more feasible to find such structures. Thus to increase the sensitivity of the biopsy, in AP cheilitis to accurately identify where the lymphoid follicles reside, based on the higher concentration of DNA in such structures and thus confirm the diagnosis. In this study, staining was positive in 85% of patients with AP cheilitis, in 14 of whom 82% lymphoid follicles were observed by histopathology. One of the pathologist’s problems in establishing the diagnosis of AP is that the main histopathological characteristics are not always identified in the submitted samples because it is not easy to clinically identify the most representative site of the lesion selected for performing a biopsy. Based on our results, we propose using toluidine blue as an auxiliary method to choose a tissue sample to facilitate the diagnosis and allow clinicians to make clinical correlations between the histopathological and therapeutic findings.
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spelling pubmed-82149032021-07-23 Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis Cedeño-Suárez, Lily Margoth Vega-Memije, María Elisa Cuevas-González, Juan Carlos Mosqueda-Taylor, Adalberto Autops Case Rep Short Communication Actinic prurigo (AP) is a type of photodermatosis that primarily affects the Latin American mestizo population. Histologically, AP cheilitis exhibits acanthosis with spongiosis and vacuolation of the basal cell layer overlying a dense lymphocytic inflammatory infiltrate that forms well-defined lymphoid follicles. Toluidine blue is a thiazide, acidophilic, and metachromatic dye used in vivo to selectively stain the acidic components of tissues such as sulfates, carboxylates, and phosphate radicals that are incorporated into DNA and RNA. It is necessary to develop a method that allows detecting, on clinical grounds the area of the lesion in which it is more feasible to find such structures. Thus to increase the sensitivity of the biopsy, in AP cheilitis to accurately identify where the lymphoid follicles reside, based on the higher concentration of DNA in such structures and thus confirm the diagnosis. In this study, staining was positive in 85% of patients with AP cheilitis, in 14 of whom 82% lymphoid follicles were observed by histopathology. One of the pathologist’s problems in establishing the diagnosis of AP is that the main histopathological characteristics are not always identified in the submitted samples because it is not easy to clinically identify the most representative site of the lesion selected for performing a biopsy. Based on our results, we propose using toluidine blue as an auxiliary method to choose a tissue sample to facilitate the diagnosis and allow clinicians to make clinical correlations between the histopathological and therapeutic findings. Hospital Universitário da Universidade de São Paulo 2021-03-12 /pmc/articles/PMC8214903/ /pubmed/34307214 http://dx.doi.org/10.4322/acr.2021.247 Text en Copyright: © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Cedeño-Suárez, Lily Margoth
Vega-Memije, María Elisa
Cuevas-González, Juan Carlos
Mosqueda-Taylor, Adalberto
Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
title Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
title_full Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
title_fullStr Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
title_full_unstemmed Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
title_short Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
title_sort value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214903/
https://www.ncbi.nlm.nih.gov/pubmed/34307214
http://dx.doi.org/10.4322/acr.2021.247
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