Cargando…

Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms

A novel negative-sense, single-stranded (ss) RNA virus was identified in a “Shennong Jinhuanghou” (SJ) grapevine showing severe chlorotic mottling symptoms by integrating high-throughput sequencing (HTS) and conventional Sanger sequencing of reverse transcription polymerase chain reaction (RT-PCR) p...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Xudong, Li, Chen, Zhang, Zunping, Ren, Fang, Hu, Guojun, Shen, Hailin, Zhang, Baodong, Dong, Yafeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215277/
https://www.ncbi.nlm.nih.gov/pubmed/34163461
http://dx.doi.org/10.3389/fmicb.2021.694601
_version_ 1783710216887468032
author Fan, Xudong
Li, Chen
Zhang, Zunping
Ren, Fang
Hu, Guojun
Shen, Hailin
Zhang, Baodong
Dong, Yafeng
author_facet Fan, Xudong
Li, Chen
Zhang, Zunping
Ren, Fang
Hu, Guojun
Shen, Hailin
Zhang, Baodong
Dong, Yafeng
author_sort Fan, Xudong
collection PubMed
description A novel negative-sense, single-stranded (ss) RNA virus was identified in a “Shennong Jinhuanghou” (SJ) grapevine showing severe chlorotic mottling symptoms by integrating high-throughput sequencing (HTS) and conventional Sanger sequencing of reverse transcription polymerase chain reaction (RT-PCR) products. The virus was provisionally named as “grapevine emaravirus A” (GEVA). GEVA had a genome comprising five genomic RNA segments, each containing a single open reading frame on the viral complementary strand and two untranslated regions with complementary 13- nt stretches at the 5′ and 3′ terminal ends. RNA1 (7,090 nt), RNA2 (2,097 nt), RNA3 (1,615 nt), and RNA4 (1,640 nt) encoded putative proteins P1–P4 that, based on their conserved motifs, were identified as the RNA-dependent RNA polymerase, glycoprotein, nucleocapsid protein, and movement protein, respectively. However, the functional role of protein P5 encoded by RNA5 (1,308 nt) could not be determined. Phylogenetic trees constructed based on amino acids of P1 to P4, allocated GEVA in clade I, together with other species-related emaraviruses. These data support the proposal that GEVA is a representative member of a novel species in the genus Emaravirus of the family Fimoviridae. Moreover, when GEVA was graft-transmitted to SJ and “Beta” grapevines, all grafted plants showed the same symptoms, similar to those observed in the source of the inoculum. This is the first report to our knowledge of an emaravirus infecting grapevine and its possible association with chlorotic mottling symptoms.
format Online
Article
Text
id pubmed-8215277
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82152772021-06-22 Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms Fan, Xudong Li, Chen Zhang, Zunping Ren, Fang Hu, Guojun Shen, Hailin Zhang, Baodong Dong, Yafeng Front Microbiol Microbiology A novel negative-sense, single-stranded (ss) RNA virus was identified in a “Shennong Jinhuanghou” (SJ) grapevine showing severe chlorotic mottling symptoms by integrating high-throughput sequencing (HTS) and conventional Sanger sequencing of reverse transcription polymerase chain reaction (RT-PCR) products. The virus was provisionally named as “grapevine emaravirus A” (GEVA). GEVA had a genome comprising five genomic RNA segments, each containing a single open reading frame on the viral complementary strand and two untranslated regions with complementary 13- nt stretches at the 5′ and 3′ terminal ends. RNA1 (7,090 nt), RNA2 (2,097 nt), RNA3 (1,615 nt), and RNA4 (1,640 nt) encoded putative proteins P1–P4 that, based on their conserved motifs, were identified as the RNA-dependent RNA polymerase, glycoprotein, nucleocapsid protein, and movement protein, respectively. However, the functional role of protein P5 encoded by RNA5 (1,308 nt) could not be determined. Phylogenetic trees constructed based on amino acids of P1 to P4, allocated GEVA in clade I, together with other species-related emaraviruses. These data support the proposal that GEVA is a representative member of a novel species in the genus Emaravirus of the family Fimoviridae. Moreover, when GEVA was graft-transmitted to SJ and “Beta” grapevines, all grafted plants showed the same symptoms, similar to those observed in the source of the inoculum. This is the first report to our knowledge of an emaravirus infecting grapevine and its possible association with chlorotic mottling symptoms. Frontiers Media S.A. 2021-06-07 /pmc/articles/PMC8215277/ /pubmed/34163461 http://dx.doi.org/10.3389/fmicb.2021.694601 Text en Copyright © 2021 Fan, Li, Zhang, Ren, Hu, Shen, Zhang and Dong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fan, Xudong
Li, Chen
Zhang, Zunping
Ren, Fang
Hu, Guojun
Shen, Hailin
Zhang, Baodong
Dong, Yafeng
Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms
title Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms
title_full Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms
title_fullStr Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms
title_full_unstemmed Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms
title_short Identification and Characterization of a Novel Emaravirus From Grapevine Showing Chlorotic Mottling Symptoms
title_sort identification and characterization of a novel emaravirus from grapevine showing chlorotic mottling symptoms
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215277/
https://www.ncbi.nlm.nih.gov/pubmed/34163461
http://dx.doi.org/10.3389/fmicb.2021.694601
work_keys_str_mv AT fanxudong identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT lichen identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT zhangzunping identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT renfang identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT huguojun identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT shenhailin identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT zhangbaodong identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms
AT dongyafeng identificationandcharacterizationofanovelemaravirusfromgrapevineshowingchloroticmottlingsymptoms