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New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response

Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying tot...

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Autores principales: Adda-Rezig, Hanane, Carron, Clémence, Pais de Barros, Jean-Paul, Choubley, Hélène, Charron, Émilie, Rérole, Anne-Laure, Laheurte, Caroline, Louvat, Pascale, Gaiffe, Émilie, Simula-Faivre, Dominique, Deckert, Valérie, Lagrost, Laurent, Saas, Philippe, Ducloux, Didier, Bamoulid, Jamal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215383/
https://www.ncbi.nlm.nih.gov/pubmed/34163471
http://dx.doi.org/10.3389/fimmu.2021.658404
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author Adda-Rezig, Hanane
Carron, Clémence
Pais de Barros, Jean-Paul
Choubley, Hélène
Charron, Émilie
Rérole, Anne-Laure
Laheurte, Caroline
Louvat, Pascale
Gaiffe, Émilie
Simula-Faivre, Dominique
Deckert, Valérie
Lagrost, Laurent
Saas, Philippe
Ducloux, Didier
Bamoulid, Jamal
author_facet Adda-Rezig, Hanane
Carron, Clémence
Pais de Barros, Jean-Paul
Choubley, Hélène
Charron, Émilie
Rérole, Anne-Laure
Laheurte, Caroline
Louvat, Pascale
Gaiffe, Émilie
Simula-Faivre, Dominique
Deckert, Valérie
Lagrost, Laurent
Saas, Philippe
Ducloux, Didier
Bamoulid, Jamal
author_sort Adda-Rezig, Hanane
collection PubMed
description Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length. This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV). We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length. Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity. Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis. To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures. Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS. TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect. Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
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spelling pubmed-82153832021-06-22 New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response Adda-Rezig, Hanane Carron, Clémence Pais de Barros, Jean-Paul Choubley, Hélène Charron, Émilie Rérole, Anne-Laure Laheurte, Caroline Louvat, Pascale Gaiffe, Émilie Simula-Faivre, Dominique Deckert, Valérie Lagrost, Laurent Saas, Philippe Ducloux, Didier Bamoulid, Jamal Front Immunol Immunology Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length. This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV). We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length. Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity. Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis. To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures. Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS. TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect. Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes. Frontiers Media S.A. 2021-06-07 /pmc/articles/PMC8215383/ /pubmed/34163471 http://dx.doi.org/10.3389/fimmu.2021.658404 Text en Copyright © 2021 Adda-Rezig, Carron, Pais de Barros, Choubley, Charron, Rérole, Laheurte, Louvat, Gaiffe, Simula-Faivre, Deckert, Lagrost, Saas, Ducloux and Bamoulid https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Adda-Rezig, Hanane
Carron, Clémence
Pais de Barros, Jean-Paul
Choubley, Hélène
Charron, Émilie
Rérole, Anne-Laure
Laheurte, Caroline
Louvat, Pascale
Gaiffe, Émilie
Simula-Faivre, Dominique
Deckert, Valérie
Lagrost, Laurent
Saas, Philippe
Ducloux, Didier
Bamoulid, Jamal
New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
title New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
title_full New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
title_fullStr New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
title_full_unstemmed New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
title_short New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
title_sort new insights on end-stage renal disease and healthy individual gut bacterial translocation: different carbon composition of lipopolysaccharides and different impact on monocyte inflammatory response
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215383/
https://www.ncbi.nlm.nih.gov/pubmed/34163471
http://dx.doi.org/10.3389/fimmu.2021.658404
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