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New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response
Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying tot...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215383/ https://www.ncbi.nlm.nih.gov/pubmed/34163471 http://dx.doi.org/10.3389/fimmu.2021.658404 |
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author | Adda-Rezig, Hanane Carron, Clémence Pais de Barros, Jean-Paul Choubley, Hélène Charron, Émilie Rérole, Anne-Laure Laheurte, Caroline Louvat, Pascale Gaiffe, Émilie Simula-Faivre, Dominique Deckert, Valérie Lagrost, Laurent Saas, Philippe Ducloux, Didier Bamoulid, Jamal |
author_facet | Adda-Rezig, Hanane Carron, Clémence Pais de Barros, Jean-Paul Choubley, Hélène Charron, Émilie Rérole, Anne-Laure Laheurte, Caroline Louvat, Pascale Gaiffe, Émilie Simula-Faivre, Dominique Deckert, Valérie Lagrost, Laurent Saas, Philippe Ducloux, Didier Bamoulid, Jamal |
author_sort | Adda-Rezig, Hanane |
collection | PubMed |
description | Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length. This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV). We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length. Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity. Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis. To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures. Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS. TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect. Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes. |
format | Online Article Text |
id | pubmed-8215383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82153832021-06-22 New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response Adda-Rezig, Hanane Carron, Clémence Pais de Barros, Jean-Paul Choubley, Hélène Charron, Émilie Rérole, Anne-Laure Laheurte, Caroline Louvat, Pascale Gaiffe, Émilie Simula-Faivre, Dominique Deckert, Valérie Lagrost, Laurent Saas, Philippe Ducloux, Didier Bamoulid, Jamal Front Immunol Immunology Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation. Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length. This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV). We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length. Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity. Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis. To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures. Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS. TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect. Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes. Frontiers Media S.A. 2021-06-07 /pmc/articles/PMC8215383/ /pubmed/34163471 http://dx.doi.org/10.3389/fimmu.2021.658404 Text en Copyright © 2021 Adda-Rezig, Carron, Pais de Barros, Choubley, Charron, Rérole, Laheurte, Louvat, Gaiffe, Simula-Faivre, Deckert, Lagrost, Saas, Ducloux and Bamoulid https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Adda-Rezig, Hanane Carron, Clémence Pais de Barros, Jean-Paul Choubley, Hélène Charron, Émilie Rérole, Anne-Laure Laheurte, Caroline Louvat, Pascale Gaiffe, Émilie Simula-Faivre, Dominique Deckert, Valérie Lagrost, Laurent Saas, Philippe Ducloux, Didier Bamoulid, Jamal New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response |
title | New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response |
title_full | New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response |
title_fullStr | New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response |
title_full_unstemmed | New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response |
title_short | New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response |
title_sort | new insights on end-stage renal disease and healthy individual gut bacterial translocation: different carbon composition of lipopolysaccharides and different impact on monocyte inflammatory response |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215383/ https://www.ncbi.nlm.nih.gov/pubmed/34163471 http://dx.doi.org/10.3389/fimmu.2021.658404 |
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