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Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells

BACKGROUND: Viral infections cause alteration in the total number of lymphocytes and their subset distribution. We aimed to study peripheral blood lymphocyte subsets in COVID-19 patients and to correlate these subsets with clinical and laboratory data, which may help in clarifying the pathogenesis t...

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Autores principales: Zahran, Asmaa M., Zahran, Zeinab Albadry M., Mady, Yasmeen H., Mahran, Essam Eldeen M.O., Rashad, Alaa, Makboul, Ahmed, Nasif, Khalid A., Abdelmaksoud, Aida A., El-Badawy, Omnia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215531/
https://www.ncbi.nlm.nih.gov/pubmed/34221400
http://dx.doi.org/10.4081/mrm.2021.758
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author Zahran, Asmaa M.
Zahran, Zeinab Albadry M.
Mady, Yasmeen H.
Mahran, Essam Eldeen M.O.
Rashad, Alaa
Makboul, Ahmed
Nasif, Khalid A.
Abdelmaksoud, Aida A.
El-Badawy, Omnia
author_facet Zahran, Asmaa M.
Zahran, Zeinab Albadry M.
Mady, Yasmeen H.
Mahran, Essam Eldeen M.O.
Rashad, Alaa
Makboul, Ahmed
Nasif, Khalid A.
Abdelmaksoud, Aida A.
El-Badawy, Omnia
author_sort Zahran, Asmaa M.
collection PubMed
description BACKGROUND: Viral infections cause alteration in the total number of lymphocytes and their subset distribution. We aimed to study peripheral blood lymphocyte subsets in COVID-19 patients and to correlate these subsets with clinical and laboratory data, which may help in clarifying the pathogenesis to develop novel diagnostic and prognostic biomarkers for COVID-19. METHODS: Twenty-six reverse-transcription polymerase chain reaction (RT-PCR) confirmed COVID-19 patients were subjected to medical history-taking and a thorough clinical examination. Laboratory tests included complete blood count, D dimer, ferritin, and C-reactive protein (CRP). Chest CT was used to diagnose COVID-19 pneumonia. Lymphocyte subsets were compared with those in 20 healthy controls using flow cytometry. RESULTS: Leucopenia, relative neutrophilia, lymphopenia, eosinopenia together with marked elevation in neutrophil/lymphocyte ratio were observed in our COVID-19 patients. A marked reduction was observed in T cells, including both CD4 and CD8 cells, natural killer (NK), and natural killer T cells (NKT). Double-positive T (DPT) cells, double-negative T (DNT) cells, and B cells were elevated in the patients relative to the other lymphocyte subsets. CONCLUSION: Immune-inflammatory parameters are of utmost importance in understanding the pathogenesis and in the provisional diagnosis of COVID-19. Yet, adequate care must be taken during their interpretation because of the vast discrepancies observed between studies even in the same locality. Further studies are needed to clarify the role of B cells, DPT, and DNT cells in the pathogenesis and control of COVID-19.
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spelling pubmed-82155312021-07-02 Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells Zahran, Asmaa M. Zahran, Zeinab Albadry M. Mady, Yasmeen H. Mahran, Essam Eldeen M.O. Rashad, Alaa Makboul, Ahmed Nasif, Khalid A. Abdelmaksoud, Aida A. El-Badawy, Omnia Multidiscip Respir Med Original Research Article BACKGROUND: Viral infections cause alteration in the total number of lymphocytes and their subset distribution. We aimed to study peripheral blood lymphocyte subsets in COVID-19 patients and to correlate these subsets with clinical and laboratory data, which may help in clarifying the pathogenesis to develop novel diagnostic and prognostic biomarkers for COVID-19. METHODS: Twenty-six reverse-transcription polymerase chain reaction (RT-PCR) confirmed COVID-19 patients were subjected to medical history-taking and a thorough clinical examination. Laboratory tests included complete blood count, D dimer, ferritin, and C-reactive protein (CRP). Chest CT was used to diagnose COVID-19 pneumonia. Lymphocyte subsets were compared with those in 20 healthy controls using flow cytometry. RESULTS: Leucopenia, relative neutrophilia, lymphopenia, eosinopenia together with marked elevation in neutrophil/lymphocyte ratio were observed in our COVID-19 patients. A marked reduction was observed in T cells, including both CD4 and CD8 cells, natural killer (NK), and natural killer T cells (NKT). Double-positive T (DPT) cells, double-negative T (DNT) cells, and B cells were elevated in the patients relative to the other lymphocyte subsets. CONCLUSION: Immune-inflammatory parameters are of utmost importance in understanding the pathogenesis and in the provisional diagnosis of COVID-19. Yet, adequate care must be taken during their interpretation because of the vast discrepancies observed between studies even in the same locality. Further studies are needed to clarify the role of B cells, DPT, and DNT cells in the pathogenesis and control of COVID-19. PAGEPress Publications, Pavia, Italy 2021-06-10 /pmc/articles/PMC8215531/ /pubmed/34221400 http://dx.doi.org/10.4081/mrm.2021.758 Text en ©Copyright: the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution NonCommercial 4.0 License (CC BY-NC 4.0).
spellingShingle Original Research Article
Zahran, Asmaa M.
Zahran, Zeinab Albadry M.
Mady, Yasmeen H.
Mahran, Essam Eldeen M.O.
Rashad, Alaa
Makboul, Ahmed
Nasif, Khalid A.
Abdelmaksoud, Aida A.
El-Badawy, Omnia
Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells
title Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells
title_full Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells
title_fullStr Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells
title_full_unstemmed Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells
title_short Differential alterations in peripheral lymphocyte subsets in COVID-19 patients: upregulation of double-positive and double-negative T cells
title_sort differential alterations in peripheral lymphocyte subsets in covid-19 patients: upregulation of double-positive and double-negative t cells
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215531/
https://www.ncbi.nlm.nih.gov/pubmed/34221400
http://dx.doi.org/10.4081/mrm.2021.758
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