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CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways

Expression of the immunoglobulin superfamily member CD155 was increased in a variety of human malignancies, but the role of CD155 in tumorigenesis and tumor development in cervical cancer has not been elucidated. In this study, immunohistochemistry and enzyme-linked immunosorbent assay analyses show...

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Autores principales: Liu, Lu, Wang, Ying, Geng, Chen, Wang, Aihong, Han, Sai, You, Xuewu, Sun, Yu, Zhang, Junhua, Lu, Wei, Zhang, Youzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216081/
https://www.ncbi.nlm.nih.gov/pubmed/34164340
http://dx.doi.org/10.3389/fonc.2021.655302
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author Liu, Lu
Wang, Ying
Geng, Chen
Wang, Aihong
Han, Sai
You, Xuewu
Sun, Yu
Zhang, Junhua
Lu, Wei
Zhang, Youzhong
author_facet Liu, Lu
Wang, Ying
Geng, Chen
Wang, Aihong
Han, Sai
You, Xuewu
Sun, Yu
Zhang, Junhua
Lu, Wei
Zhang, Youzhong
author_sort Liu, Lu
collection PubMed
description Expression of the immunoglobulin superfamily member CD155 was increased in a variety of human malignancies, but the role of CD155 in tumorigenesis and tumor development in cervical cancer has not been elucidated. In this study, immunohistochemistry and enzyme-linked immunosorbent assay analyses showed that CD155 expression gradually increases with the degree of cervical lesions. In vitro and in vivo, reducing the expression of CD155 inhibited cell proliferation, cell viability and tumor formation and arrested the cell cycle in G0/G1 phase. Antibody array-based profiling of protein phosphorylation revealed that CD155 knockdown can inhibited the AKT/mTOR/NF-κB pathway and activated autophagy and apoptosis; the opposite effects were observed upon CD155 has overexpression. We proved that there is an interaction between CD155 and AKT by immunoprecipitation. We further confirmed the mechanism between CD155 and AKT/mTOR/NF-κB through rescue experiments. AKT knockdown reversed the anti-apoptotic effects and activation of the AKT/mTOR/NF-κB pathway induced by CD155 overexpression. Our research demonstrated that CD155 can interact with AKT to form a complex, activates the AKT/mTOR/NF-κB pathway and inhibit autophagy and apoptosis. Thus, CD155 is a potential screening and therapeutic biomarker for cervical cancer.
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spelling pubmed-82160812021-06-22 CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways Liu, Lu Wang, Ying Geng, Chen Wang, Aihong Han, Sai You, Xuewu Sun, Yu Zhang, Junhua Lu, Wei Zhang, Youzhong Front Oncol Oncology Expression of the immunoglobulin superfamily member CD155 was increased in a variety of human malignancies, but the role of CD155 in tumorigenesis and tumor development in cervical cancer has not been elucidated. In this study, immunohistochemistry and enzyme-linked immunosorbent assay analyses showed that CD155 expression gradually increases with the degree of cervical lesions. In vitro and in vivo, reducing the expression of CD155 inhibited cell proliferation, cell viability and tumor formation and arrested the cell cycle in G0/G1 phase. Antibody array-based profiling of protein phosphorylation revealed that CD155 knockdown can inhibited the AKT/mTOR/NF-κB pathway and activated autophagy and apoptosis; the opposite effects were observed upon CD155 has overexpression. We proved that there is an interaction between CD155 and AKT by immunoprecipitation. We further confirmed the mechanism between CD155 and AKT/mTOR/NF-κB through rescue experiments. AKT knockdown reversed the anti-apoptotic effects and activation of the AKT/mTOR/NF-κB pathway induced by CD155 overexpression. Our research demonstrated that CD155 can interact with AKT to form a complex, activates the AKT/mTOR/NF-κB pathway and inhibit autophagy and apoptosis. Thus, CD155 is a potential screening and therapeutic biomarker for cervical cancer. Frontiers Media S.A. 2021-06-07 /pmc/articles/PMC8216081/ /pubmed/34164340 http://dx.doi.org/10.3389/fonc.2021.655302 Text en Copyright © 2021 Liu, Wang, Geng, Wang, Han, You, Sun, Zhang, Lu and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Liu, Lu
Wang, Ying
Geng, Chen
Wang, Aihong
Han, Sai
You, Xuewu
Sun, Yu
Zhang, Junhua
Lu, Wei
Zhang, Youzhong
CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways
title CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways
title_full CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways
title_fullStr CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways
title_full_unstemmed CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways
title_short CD155 Promotes the Progression of Cervical Cancer Cells Through AKT/mTOR and NF-κB Pathways
title_sort cd155 promotes the progression of cervical cancer cells through akt/mtor and nf-κb pathways
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216081/
https://www.ncbi.nlm.nih.gov/pubmed/34164340
http://dx.doi.org/10.3389/fonc.2021.655302
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