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Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors

Plants MADS-domain/AGL proteins constitute a large transcription factor (TF) family that controls the development of almost every plant organ. We performed a phylogeny of (ca. 500) MADS-domain proteins from Arabidopsis and four legume species. We identified clades with Arabidopsis MADS-domain protei...

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Autores principales: Ayra, Litzy, Reyero-Saavedra, María del Rocio, Isidra-Arellano, Mariel C., Lozano, Luis, Ramírez, Mario, Leija, Alfonso, Fuentes, Sara-Isabel, Girard, Lourdes, Valdés-López, Oswaldo, Hernández, Georgina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216239/
https://www.ncbi.nlm.nih.gov/pubmed/34163511
http://dx.doi.org/10.3389/fpls.2021.679463
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author Ayra, Litzy
Reyero-Saavedra, María del Rocio
Isidra-Arellano, Mariel C.
Lozano, Luis
Ramírez, Mario
Leija, Alfonso
Fuentes, Sara-Isabel
Girard, Lourdes
Valdés-López, Oswaldo
Hernández, Georgina
author_facet Ayra, Litzy
Reyero-Saavedra, María del Rocio
Isidra-Arellano, Mariel C.
Lozano, Luis
Ramírez, Mario
Leija, Alfonso
Fuentes, Sara-Isabel
Girard, Lourdes
Valdés-López, Oswaldo
Hernández, Georgina
author_sort Ayra, Litzy
collection PubMed
description Plants MADS-domain/AGL proteins constitute a large transcription factor (TF) family that controls the development of almost every plant organ. We performed a phylogeny of (ca. 500) MADS-domain proteins from Arabidopsis and four legume species. We identified clades with Arabidopsis MADS-domain proteins known to participate in root development that grouped legume MADS-proteins with similar high expression in roots and nodules. In this work, we analyzed the role of AGL transcription factors in the common bean (Phaseolus vulgaris) – Rhizobium etli N-fixing symbiosis. Sixteen P. vulgaris AGL genes (PvAGL), out of 93 family members, are expressed – at different levels – in roots and nodules. From there, we selected the PvAGL gene denominated PvFUL-like for overexpression or silencing in composite plants, with transgenic roots and nodules, that were used for phenotypic analysis upon inoculation with Rhizobium etli. Because of sequence identity in the DNA sequence used for RNAi-FUL-like construct, roots, and nodules expressing this construct -referred to as RNAi_AGL- showed lower expression of other five PvAGL genes highly expressed in roots/nodules. Contrasting with PvFUL-like overexpressing plants, rhizobia-inoculated plants expressing the RNAi_AGL silencing construct presented affection in the generation and growth of transgenic roots from composite plants, both under non-inoculated or rhizobia-inoculated condition. Furthermore, the rhizobia-inoculated plants showed decreased rhizobial infection concomitant with the lower expression level of early symbiotic genes and increased number of small, ineffective nodules that indicate an alteration in the autoregulation of the nodulation symbiotic process. We propose that the positive effects of PvAGL TF in the rhizobia symbiotic processes result from its potential interplay with NIN, the master symbiotic TF regulator, that showed a CArG-box consensus DNA sequence recognized for DNA binding of AGL TF and presented an increased or decreased expression level in roots from non-inoculated plants transformed with OE_FUL or RNAi_AGL construct, respectively. Our work contributes to defining novel transcriptional regulators for the common bean – rhizobia N-fixing symbiosis, a relevant process for sustainable agriculture.
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spelling pubmed-82162392021-06-22 Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors Ayra, Litzy Reyero-Saavedra, María del Rocio Isidra-Arellano, Mariel C. Lozano, Luis Ramírez, Mario Leija, Alfonso Fuentes, Sara-Isabel Girard, Lourdes Valdés-López, Oswaldo Hernández, Georgina Front Plant Sci Plant Science Plants MADS-domain/AGL proteins constitute a large transcription factor (TF) family that controls the development of almost every plant organ. We performed a phylogeny of (ca. 500) MADS-domain proteins from Arabidopsis and four legume species. We identified clades with Arabidopsis MADS-domain proteins known to participate in root development that grouped legume MADS-proteins with similar high expression in roots and nodules. In this work, we analyzed the role of AGL transcription factors in the common bean (Phaseolus vulgaris) – Rhizobium etli N-fixing symbiosis. Sixteen P. vulgaris AGL genes (PvAGL), out of 93 family members, are expressed – at different levels – in roots and nodules. From there, we selected the PvAGL gene denominated PvFUL-like for overexpression or silencing in composite plants, with transgenic roots and nodules, that were used for phenotypic analysis upon inoculation with Rhizobium etli. Because of sequence identity in the DNA sequence used for RNAi-FUL-like construct, roots, and nodules expressing this construct -referred to as RNAi_AGL- showed lower expression of other five PvAGL genes highly expressed in roots/nodules. Contrasting with PvFUL-like overexpressing plants, rhizobia-inoculated plants expressing the RNAi_AGL silencing construct presented affection in the generation and growth of transgenic roots from composite plants, both under non-inoculated or rhizobia-inoculated condition. Furthermore, the rhizobia-inoculated plants showed decreased rhizobial infection concomitant with the lower expression level of early symbiotic genes and increased number of small, ineffective nodules that indicate an alteration in the autoregulation of the nodulation symbiotic process. We propose that the positive effects of PvAGL TF in the rhizobia symbiotic processes result from its potential interplay with NIN, the master symbiotic TF regulator, that showed a CArG-box consensus DNA sequence recognized for DNA binding of AGL TF and presented an increased or decreased expression level in roots from non-inoculated plants transformed with OE_FUL or RNAi_AGL construct, respectively. Our work contributes to defining novel transcriptional regulators for the common bean – rhizobia N-fixing symbiosis, a relevant process for sustainable agriculture. Frontiers Media S.A. 2021-06-07 /pmc/articles/PMC8216239/ /pubmed/34163511 http://dx.doi.org/10.3389/fpls.2021.679463 Text en Copyright © 2021 Ayra, Reyero-Saavedra, Isidra-Arellano, Lozano, Ramírez, Leija, Fuentes, Girard, Valdés-López and Hernández. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ayra, Litzy
Reyero-Saavedra, María del Rocio
Isidra-Arellano, Mariel C.
Lozano, Luis
Ramírez, Mario
Leija, Alfonso
Fuentes, Sara-Isabel
Girard, Lourdes
Valdés-López, Oswaldo
Hernández, Georgina
Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
title Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
title_full Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
title_fullStr Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
title_full_unstemmed Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
title_short Control of the Rhizobia Nitrogen-Fixing Symbiosis by Common Bean MADS-Domain/AGL Transcription Factors
title_sort control of the rhizobia nitrogen-fixing symbiosis by common bean mads-domain/agl transcription factors
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216239/
https://www.ncbi.nlm.nih.gov/pubmed/34163511
http://dx.doi.org/10.3389/fpls.2021.679463
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