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Optimizing protein V untranslated region sequence in M13 phage for increased production of single-stranded DNA for origami

DNA origami requires long scaffold DNA to be aligned with the guidance of short staple DNA strands. Scaffold DNA is produced in Escherichia coli as a form of the M13 bacteriophage by rolling circle amplification (RCA). This study shows that RCA can be reconfigured by reducing phage protein V (pV) ex...

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Detalles Bibliográficos
Autores principales:	Lee, Bo-Young, Lee, Jaewon, Ahn, Dong June, Lee, Seungwoo, Oh, Min-Kyu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216270/ https://www.ncbi.nlm.nih.gov/pubmed/34110422 http://dx.doi.org/10.1093/nar/gkab455

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