Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells

The genetic code of mammalian cells can be expanded to allow the incorporation of non-canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an orthogonal pyrrolysyl-tRNA synthetase (PylRS)/tRNA(Pyl)(CUA) (PylT) pair. However, the feasibility of this approach is substanti...

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Detalles Bibliográficos
Autores principales: Bartoschek, Michael D, Ugur, Enes, Nguyen, Tuan‐Anh, Rodschinka, Geraldine, Wierer, Michael, Lang, Kathrin, Bultmann, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216290/
https://www.ncbi.nlm.nih.gov/pubmed/33684219
http://dx.doi.org/10.1093/nar/gkab132

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216290/
https://www.ncbi.nlm.nih.gov/pubmed/33684219
http://dx.doi.org/10.1093/nar/gkab132

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