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Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells

The genetic code of mammalian cells can be expanded to allow the incorporation of non-canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an orthogonal pyrrolysyl-tRNA synthetase (PylRS)/tRNA(Pyl)(CUA) (PylT) pair. However, the feasibility of this approach is substanti...

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Detalles Bibliográficos
Autores principales:	Bartoschek, Michael D, Ugur, Enes, Nguyen, Tuan‐Anh, Rodschinka, Geraldine, Wierer, Michael, Lang, Kathrin, Bultmann, Sebastian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216290/ https://www.ncbi.nlm.nih.gov/pubmed/33684219 http://dx.doi.org/10.1093/nar/gkab132

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