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In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration
The purpose of this study is to evaluate in vitro and in vivo antitumor efficacy and subacute toxicity of triptolide (TP) prodrug, a conjugate between TP and carboxymethyl chitosan (CC). The CCTP conjugate contained 4∼ wt % TP and displayed excellent aqueous solubility (5 mg/mL) as compared to the n...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216443/ https://www.ncbi.nlm.nih.gov/pubmed/32508161 http://dx.doi.org/10.1080/10717544.2020.1770370 |
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author | Zeng, Huahui Zhu, Xin Tian, Qikang Yan, Yinyin Zhang, Lan Yan, Min Li, Ruiqin Li, Xiaofang Wang, Guoqiang Ma, Jinlian Su, Yufang Zhang, Xiangbo Ma, Linyu Zhang, Zhenqiang Wu, Xiangxiang |
author_facet | Zeng, Huahui Zhu, Xin Tian, Qikang Yan, Yinyin Zhang, Lan Yan, Min Li, Ruiqin Li, Xiaofang Wang, Guoqiang Ma, Jinlian Su, Yufang Zhang, Xiangbo Ma, Linyu Zhang, Zhenqiang Wu, Xiangxiang |
author_sort | Zeng, Huahui |
collection | PubMed |
description | The purpose of this study is to evaluate in vitro and in vivo antitumor efficacy and subacute toxicity of triptolide (TP) prodrug, a conjugate between TP and carboxymethyl chitosan (CC). The CCTP conjugate contained 4∼ wt % TP and displayed excellent aqueous solubility (5 mg/mL) as compared to the native TP (17 μg/mL). In vitro cytotoxicity of CCTP conjugate was evaluated by CCK8 assay against human pancreatic cancer (PC) cell lines, showing comparable the half maximal inhibitory concentration (IC(50)) values to the parent TP. In a mouse model of PC (BxPC-3), the CCTP conjugate administered orally (at dose levels as low as 0.2 mg TP equivalent/kg) showed comparable efficacy in reducing or eliminating xenograft tumor to the same dose of TP, but exhibited much lower subacute toxicity as seen in body weight loss and hematological toxicity. |
format | Online Article Text |
id | pubmed-8216443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-82164432021-07-06 In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration Zeng, Huahui Zhu, Xin Tian, Qikang Yan, Yinyin Zhang, Lan Yan, Min Li, Ruiqin Li, Xiaofang Wang, Guoqiang Ma, Jinlian Su, Yufang Zhang, Xiangbo Ma, Linyu Zhang, Zhenqiang Wu, Xiangxiang Drug Deliv Article The purpose of this study is to evaluate in vitro and in vivo antitumor efficacy and subacute toxicity of triptolide (TP) prodrug, a conjugate between TP and carboxymethyl chitosan (CC). The CCTP conjugate contained 4∼ wt % TP and displayed excellent aqueous solubility (5 mg/mL) as compared to the native TP (17 μg/mL). In vitro cytotoxicity of CCTP conjugate was evaluated by CCK8 assay against human pancreatic cancer (PC) cell lines, showing comparable the half maximal inhibitory concentration (IC(50)) values to the parent TP. In a mouse model of PC (BxPC-3), the CCTP conjugate administered orally (at dose levels as low as 0.2 mg TP equivalent/kg) showed comparable efficacy in reducing or eliminating xenograft tumor to the same dose of TP, but exhibited much lower subacute toxicity as seen in body weight loss and hematological toxicity. Taylor & Francis 2020-06-08 /pmc/articles/PMC8216443/ /pubmed/32508161 http://dx.doi.org/10.1080/10717544.2020.1770370 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Zeng, Huahui Zhu, Xin Tian, Qikang Yan, Yinyin Zhang, Lan Yan, Min Li, Ruiqin Li, Xiaofang Wang, Guoqiang Ma, Jinlian Su, Yufang Zhang, Xiangbo Ma, Linyu Zhang, Zhenqiang Wu, Xiangxiang In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
title | In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
title_full | In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
title_fullStr | In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
title_full_unstemmed | In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
title_short | In vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
title_sort | in vivo antitumor effects of carboxymethyl chitosan-conjugated triptolide after oral administration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216443/ https://www.ncbi.nlm.nih.gov/pubmed/32508161 http://dx.doi.org/10.1080/10717544.2020.1770370 |
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