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Comprehensive analysis of bacteriocins in Streptococcus mutans
Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on th...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217173/ https://www.ncbi.nlm.nih.gov/pubmed/34155274 http://dx.doi.org/10.1038/s41598-021-92370-1 |
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author | Watanabe, Atsuko Kawada-Matsuo, Miki Le, Mi Nguyen-Tra Hisatsune, Junzo Oogai, Yuichi Nakano, Yoshio Nakata, Masanobu Miyawaki, Shouichi Sugai, Motoyuki Komatsuzawa, Hitoshi |
author_facet | Watanabe, Atsuko Kawada-Matsuo, Miki Le, Mi Nguyen-Tra Hisatsune, Junzo Oogai, Yuichi Nakano, Yoshio Nakata, Masanobu Miyawaki, Shouichi Sugai, Motoyuki Komatsuzawa, Hitoshi |
author_sort | Watanabe, Atsuko |
collection | PubMed |
description | Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on the genome analysis, the distribution of each bacteriocin gene (mutacins I-IV, K8 and Smb) was investigated. We found 17, 5, and 2 strains showing 100% matches with mutacin I, mutacin II and mutacin III, respectively. Five mutacin III-positive strains had 2 mismatches compared to mature mutacin III. In 67 mutacin IV-positive strains, 38 strains showed 100% match with mutacin IV, while 29 strains showed some variations. In 23 mutacin K8- and 32 mutacin Smb-positive strains, all except one mutacin K8-positive strain showed 100% match with the mature peptides. Among 125 strains, 84 (65.1%), 26 (20.2%), and 5 (3.9%) strains were positive for one, two and three bacteriocin genes, respectively. Then, the antibacterial activity against oral streptococci and other oral bacterial species was investigated by using bacteriocin gene single-positive strains. Each bacteriocin gene-positive strain showed a different pattern of antibacterial activity. These results speculate that individual S. mutans strains may affect the bacterial composition of dental plaques. |
format | Online Article Text |
id | pubmed-8217173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82171732021-06-22 Comprehensive analysis of bacteriocins in Streptococcus mutans Watanabe, Atsuko Kawada-Matsuo, Miki Le, Mi Nguyen-Tra Hisatsune, Junzo Oogai, Yuichi Nakano, Yoshio Nakata, Masanobu Miyawaki, Shouichi Sugai, Motoyuki Komatsuzawa, Hitoshi Sci Rep Article Streptococcus mutans produces bacteriocins that show antibacterial activity against several bacteria. However, comprehensive analysis of these bacteriocins has not been well done. In this study, we isolated 125 S. mutans strains from volunteers and determined their whole genome sequence. Based on the genome analysis, the distribution of each bacteriocin gene (mutacins I-IV, K8 and Smb) was investigated. We found 17, 5, and 2 strains showing 100% matches with mutacin I, mutacin II and mutacin III, respectively. Five mutacin III-positive strains had 2 mismatches compared to mature mutacin III. In 67 mutacin IV-positive strains, 38 strains showed 100% match with mutacin IV, while 29 strains showed some variations. In 23 mutacin K8- and 32 mutacin Smb-positive strains, all except one mutacin K8-positive strain showed 100% match with the mature peptides. Among 125 strains, 84 (65.1%), 26 (20.2%), and 5 (3.9%) strains were positive for one, two and three bacteriocin genes, respectively. Then, the antibacterial activity against oral streptococci and other oral bacterial species was investigated by using bacteriocin gene single-positive strains. Each bacteriocin gene-positive strain showed a different pattern of antibacterial activity. These results speculate that individual S. mutans strains may affect the bacterial composition of dental plaques. Nature Publishing Group UK 2021-06-21 /pmc/articles/PMC8217173/ /pubmed/34155274 http://dx.doi.org/10.1038/s41598-021-92370-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Watanabe, Atsuko Kawada-Matsuo, Miki Le, Mi Nguyen-Tra Hisatsune, Junzo Oogai, Yuichi Nakano, Yoshio Nakata, Masanobu Miyawaki, Shouichi Sugai, Motoyuki Komatsuzawa, Hitoshi Comprehensive analysis of bacteriocins in Streptococcus mutans |
title | Comprehensive analysis of bacteriocins in Streptococcus mutans |
title_full | Comprehensive analysis of bacteriocins in Streptococcus mutans |
title_fullStr | Comprehensive analysis of bacteriocins in Streptococcus mutans |
title_full_unstemmed | Comprehensive analysis of bacteriocins in Streptococcus mutans |
title_short | Comprehensive analysis of bacteriocins in Streptococcus mutans |
title_sort | comprehensive analysis of bacteriocins in streptococcus mutans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217173/ https://www.ncbi.nlm.nih.gov/pubmed/34155274 http://dx.doi.org/10.1038/s41598-021-92370-1 |
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