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Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis

Angelica sinensis (Oliv.) Diels root part is an integral component of traditional Chinese medicine, widely prescribed to improve blood circulation and blood stasis. However, early bolting of A. sinensis compromises the quality of the roots and hence is a major limitation for yield of medicinal mater...

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Autores principales: Gao, Xue, Guo, Fengxia, Chen, Yuan, Bai, Gang, Liu, Yuxiao, Jin, Jianqin, Wang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217430/
https://www.ncbi.nlm.nih.gov/pubmed/34155325
http://dx.doi.org/10.1038/s41598-021-92494-4
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author Gao, Xue
Guo, Fengxia
Chen, Yuan
Bai, Gang
Liu, Yuxiao
Jin, Jianqin
Wang, Qing
author_facet Gao, Xue
Guo, Fengxia
Chen, Yuan
Bai, Gang
Liu, Yuxiao
Jin, Jianqin
Wang, Qing
author_sort Gao, Xue
collection PubMed
description Angelica sinensis (Oliv.) Diels root part is an integral component of traditional Chinese medicine, widely prescribed to improve blood circulation and blood stasis. However, early bolting of A. sinensis compromises the quality of the roots and hence is a major limitation for yield of medicinal materials. To date, little information about the molecular mechanisms underlying bolting is available for this important medicinal plant. To identify genes putatively involved in early bolting, we have conducted the transcriptome analysis of the shoot tips of the early-bolting plants and non-bolting (normal) plants of A. sinensis, respectively, using a combination of third-generation sequencing and next-generation sequencing. A total of 43,438 non-redundant transcripts were collected and 475 unique differentially expressed genes (DEGs) were identified. Gene annotation and functional analyses revealed that DEGs were highly involved in plant hormone signaling and biosynthesis pathways, three main flowering pathways, pollen formation, and very-long-chain fatty acids biosynthesis pathways. The levels of endogenous hormones were also changed significantly in the early bolting stage of A. sinensis. This study provided new insights into the transcriptomic control of early bolting in A. sinensis, which could be further applied to enhance the yield of medicinally important raw materials.
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spelling pubmed-82174302021-06-22 Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis Gao, Xue Guo, Fengxia Chen, Yuan Bai, Gang Liu, Yuxiao Jin, Jianqin Wang, Qing Sci Rep Article Angelica sinensis (Oliv.) Diels root part is an integral component of traditional Chinese medicine, widely prescribed to improve blood circulation and blood stasis. However, early bolting of A. sinensis compromises the quality of the roots and hence is a major limitation for yield of medicinal materials. To date, little information about the molecular mechanisms underlying bolting is available for this important medicinal plant. To identify genes putatively involved in early bolting, we have conducted the transcriptome analysis of the shoot tips of the early-bolting plants and non-bolting (normal) plants of A. sinensis, respectively, using a combination of third-generation sequencing and next-generation sequencing. A total of 43,438 non-redundant transcripts were collected and 475 unique differentially expressed genes (DEGs) were identified. Gene annotation and functional analyses revealed that DEGs were highly involved in plant hormone signaling and biosynthesis pathways, three main flowering pathways, pollen formation, and very-long-chain fatty acids biosynthesis pathways. The levels of endogenous hormones were also changed significantly in the early bolting stage of A. sinensis. This study provided new insights into the transcriptomic control of early bolting in A. sinensis, which could be further applied to enhance the yield of medicinally important raw materials. Nature Publishing Group UK 2021-06-21 /pmc/articles/PMC8217430/ /pubmed/34155325 http://dx.doi.org/10.1038/s41598-021-92494-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gao, Xue
Guo, Fengxia
Chen, Yuan
Bai, Gang
Liu, Yuxiao
Jin, Jianqin
Wang, Qing
Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_full Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_fullStr Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_full_unstemmed Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_short Full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal Angelica sinensis
title_sort full-length transcriptome analysis provides new insights into the early bolting occurrence in medicinal angelica sinensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217430/
https://www.ncbi.nlm.nih.gov/pubmed/34155325
http://dx.doi.org/10.1038/s41598-021-92494-4
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