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A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation

Networks of transcriptional and post-transcriptional regulators are critical for bacterial survival and adaptation to environmental stressors. While transcriptional regulators provide rapid activation and/or repression of a wide-network of genes, post-transcriptional regulators, such as small RNAs (...

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Autores principales: Villa, Jordan K., Han, Runhua, Tsai, Chen-Hsun, Chen, Angela, Sweet, Philip, Franco, Gabriela, Vaezian, Respina, Tkavc, Rok, Daly, Michael J., Contreras, Lydia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217566/
https://www.ncbi.nlm.nih.gov/pubmed/34155239
http://dx.doi.org/10.1038/s41598-021-91335-8
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author Villa, Jordan K.
Han, Runhua
Tsai, Chen-Hsun
Chen, Angela
Sweet, Philip
Franco, Gabriela
Vaezian, Respina
Tkavc, Rok
Daly, Michael J.
Contreras, Lydia M.
author_facet Villa, Jordan K.
Han, Runhua
Tsai, Chen-Hsun
Chen, Angela
Sweet, Philip
Franco, Gabriela
Vaezian, Respina
Tkavc, Rok
Daly, Michael J.
Contreras, Lydia M.
author_sort Villa, Jordan K.
collection PubMed
description Networks of transcriptional and post-transcriptional regulators are critical for bacterial survival and adaptation to environmental stressors. While transcriptional regulators provide rapid activation and/or repression of a wide-network of genes, post-transcriptional regulators, such as small RNAs (sRNAs), are also important to fine-tune gene expression. However, the mechanisms of sRNAs remain poorly understood, especially in less-studied bacteria. Deinococcus radiodurans is a gram-positive bacterium resistant to extreme levels of ionizing radiation (IR). Although multiple unique regulatory systems (e.g., the Radiation and Desiccation Response (RDR)) have been identified in this organism, the role of post-transcriptional regulators has not been characterized within the IR response. In this study, we have characterized an sRNA, PprS (formerly Dsr2), as a post-transcriptional coordinator of IR recovery in D. radiodurans. PprS showed differential expression specifically under IR and knockdown of PprS resulted in reduced survival and growth under IR, suggesting its importance in regulating post-radiation recovery. We determined a number of potential RNA targets involved in several pathways including translation and DNA repair. Specifically, we confirmed that PprS binds within the coding region to stabilize the pprM (DR_0907) transcript, a RDR modulator. Overall, these results are the first to present an additional layer of sRNA-based control in DNA repair pathways associated with bacterial radioresistance.
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spelling pubmed-82175662021-06-22 A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation Villa, Jordan K. Han, Runhua Tsai, Chen-Hsun Chen, Angela Sweet, Philip Franco, Gabriela Vaezian, Respina Tkavc, Rok Daly, Michael J. Contreras, Lydia M. Sci Rep Article Networks of transcriptional and post-transcriptional regulators are critical for bacterial survival and adaptation to environmental stressors. While transcriptional regulators provide rapid activation and/or repression of a wide-network of genes, post-transcriptional regulators, such as small RNAs (sRNAs), are also important to fine-tune gene expression. However, the mechanisms of sRNAs remain poorly understood, especially in less-studied bacteria. Deinococcus radiodurans is a gram-positive bacterium resistant to extreme levels of ionizing radiation (IR). Although multiple unique regulatory systems (e.g., the Radiation and Desiccation Response (RDR)) have been identified in this organism, the role of post-transcriptional regulators has not been characterized within the IR response. In this study, we have characterized an sRNA, PprS (formerly Dsr2), as a post-transcriptional coordinator of IR recovery in D. radiodurans. PprS showed differential expression specifically under IR and knockdown of PprS resulted in reduced survival and growth under IR, suggesting its importance in regulating post-radiation recovery. We determined a number of potential RNA targets involved in several pathways including translation and DNA repair. Specifically, we confirmed that PprS binds within the coding region to stabilize the pprM (DR_0907) transcript, a RDR modulator. Overall, these results are the first to present an additional layer of sRNA-based control in DNA repair pathways associated with bacterial radioresistance. Nature Publishing Group UK 2021-06-21 /pmc/articles/PMC8217566/ /pubmed/34155239 http://dx.doi.org/10.1038/s41598-021-91335-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Villa, Jordan K.
Han, Runhua
Tsai, Chen-Hsun
Chen, Angela
Sweet, Philip
Franco, Gabriela
Vaezian, Respina
Tkavc, Rok
Daly, Michael J.
Contreras, Lydia M.
A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
title A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
title_full A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
title_fullStr A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
title_full_unstemmed A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
title_short A small RNA regulates pprM, a modulator of pleiotropic proteins promoting DNA repair, in Deinococcus radiodurans under ionizing radiation
title_sort small rna regulates pprm, a modulator of pleiotropic proteins promoting dna repair, in deinococcus radiodurans under ionizing radiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217566/
https://www.ncbi.nlm.nih.gov/pubmed/34155239
http://dx.doi.org/10.1038/s41598-021-91335-8
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