Cargando…

Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice

PURPOSE: In the present study, meningococcal serogroup B outer membrane vesicles (OMVs) were associated with bilayer fragments of a cationic lipid, dioctadecyldimethylammonium (DDA-BF), used as adjuvant, in an antigenic preparation tested in adult female outbred mice. This adjuvant was compared to t...

Descripción completa

Detalles Bibliográficos
Autores principales: Rinaldi, Fabiana Mahylowski, Gaspar, Emanuelle Baldo, Brito, Luciana Tendolini, Gaspari, Elizabeth De
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217576/
https://www.ncbi.nlm.nih.gov/pubmed/34222123
http://dx.doi.org/10.7774/cevr.2021.10.2.106
_version_ 1783710620829351936
author Rinaldi, Fabiana Mahylowski
Gaspar, Emanuelle Baldo
Brito, Luciana Tendolini
Gaspari, Elizabeth De
author_facet Rinaldi, Fabiana Mahylowski
Gaspar, Emanuelle Baldo
Brito, Luciana Tendolini
Gaspari, Elizabeth De
author_sort Rinaldi, Fabiana Mahylowski
collection PubMed
description PURPOSE: In the present study, meningococcal serogroup B outer membrane vesicles (OMVs) were associated with bilayer fragments of a cationic lipid, dioctadecyldimethylammonium (DDA-BF), used as adjuvant, in an antigenic preparation tested in adult female outbred mice. This adjuvant was compared to the traditional adjuvant aluminum hydroxide. MATERIALS AND METHODS: The potential in generating humoral response was evaluated by enzyme-linked immunosorbent assay (ELISA). Individual serum was collected and immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b were quantified. Analyses were carried out 15 and 60 days after immunization. Antibodies avidity index were also analyzed by ELISA. Immunoblot and dot-ELISA were carried out to evaluate specific reaction for homologous strains and cross-reactive antigens present in other meningococcal strains isolated in 2011–2012 year, in Brazil. Delayed type hypersensitivity was used as indicative of cellular immunity and compared between two experimental groups, 24 hours after homologous strain challenge. RESULTS: The OMVs of Neisseria meningitidis, and N. lactamica (related species) were characterized by electrophoretic separation of proteins in 13% polyacrylamide gel. The strains presented antigens in the range of 8 to 130 kDa, showing a heterogeneous protein migration pattern. In the group immunized with OMVs/DDA-BF, we found no significant production of total IgG 15 days after the first immunization. On the other hand, 60 days after first immunization both adjuvants act benefiting total IgG production similarly. The antibodies of the IgG isotype produced by animals immunized after one or two doses after first immunization, showed intermediate and high avidity, independent on the adjuvant used. In both experimental groups the swelling of the footpads was significantly higher than those of the controls, suggesting that only one dose was enough to stimulate the generation of cellular immunity. CONCLUSION: The use of this cationic adjuvant for N. meningitidis OMVs preparation revealed good potential for future new antigen preparation for N. meningitidis vaccine.
format Online
Article
Text
id pubmed-8217576
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher The Korean Vaccine Society
record_format MEDLINE/PubMed
spelling pubmed-82175762021-07-01 Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice Rinaldi, Fabiana Mahylowski Gaspar, Emanuelle Baldo Brito, Luciana Tendolini Gaspari, Elizabeth De Clin Exp Vaccine Res Original Article PURPOSE: In the present study, meningococcal serogroup B outer membrane vesicles (OMVs) were associated with bilayer fragments of a cationic lipid, dioctadecyldimethylammonium (DDA-BF), used as adjuvant, in an antigenic preparation tested in adult female outbred mice. This adjuvant was compared to the traditional adjuvant aluminum hydroxide. MATERIALS AND METHODS: The potential in generating humoral response was evaluated by enzyme-linked immunosorbent assay (ELISA). Individual serum was collected and immunoglobulin G (IgG), IgG1, IgG2a, and IgG2b were quantified. Analyses were carried out 15 and 60 days after immunization. Antibodies avidity index were also analyzed by ELISA. Immunoblot and dot-ELISA were carried out to evaluate specific reaction for homologous strains and cross-reactive antigens present in other meningococcal strains isolated in 2011–2012 year, in Brazil. Delayed type hypersensitivity was used as indicative of cellular immunity and compared between two experimental groups, 24 hours after homologous strain challenge. RESULTS: The OMVs of Neisseria meningitidis, and N. lactamica (related species) were characterized by electrophoretic separation of proteins in 13% polyacrylamide gel. The strains presented antigens in the range of 8 to 130 kDa, showing a heterogeneous protein migration pattern. In the group immunized with OMVs/DDA-BF, we found no significant production of total IgG 15 days after the first immunization. On the other hand, 60 days after first immunization both adjuvants act benefiting total IgG production similarly. The antibodies of the IgG isotype produced by animals immunized after one or two doses after first immunization, showed intermediate and high avidity, independent on the adjuvant used. In both experimental groups the swelling of the footpads was significantly higher than those of the controls, suggesting that only one dose was enough to stimulate the generation of cellular immunity. CONCLUSION: The use of this cationic adjuvant for N. meningitidis OMVs preparation revealed good potential for future new antigen preparation for N. meningitidis vaccine. The Korean Vaccine Society 2021-05 2021-05-31 /pmc/articles/PMC8217576/ /pubmed/34222123 http://dx.doi.org/10.7774/cevr.2021.10.2.106 Text en © Korean Vaccine Society. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rinaldi, Fabiana Mahylowski
Gaspar, Emanuelle Baldo
Brito, Luciana Tendolini
Gaspari, Elizabeth De
Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice
title Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice
title_full Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice
title_fullStr Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice
title_full_unstemmed Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice
title_short Immunogenicity of antigens from outer membrane vesicles of Neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in Swiss adult mice
title_sort immunogenicity of antigens from outer membrane vesicles of neisseria meningitidis associated with bilayer fragment of dioctadecyldimethylammonium in swiss adult mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217576/
https://www.ncbi.nlm.nih.gov/pubmed/34222123
http://dx.doi.org/10.7774/cevr.2021.10.2.106
work_keys_str_mv AT rinaldifabianamahylowski immunogenicityofantigensfromoutermembranevesiclesofneisseriameningitidisassociatedwithbilayerfragmentofdioctadecyldimethylammoniuminswissadultmice
AT gasparemanuellebaldo immunogenicityofantigensfromoutermembranevesiclesofneisseriameningitidisassociatedwithbilayerfragmentofdioctadecyldimethylammoniuminswissadultmice
AT britolucianatendolini immunogenicityofantigensfromoutermembranevesiclesofneisseriameningitidisassociatedwithbilayerfragmentofdioctadecyldimethylammoniuminswissadultmice
AT gasparielizabethde immunogenicityofantigensfromoutermembranevesiclesofneisseriameningitidisassociatedwithbilayerfragmentofdioctadecyldimethylammoniuminswissadultmice