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Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
PURPOSE: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). MATERIALS AND METHODS: Six groups of female Sprague Dawley albino rats (10/g...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Vaccine Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217580/ https://www.ncbi.nlm.nih.gov/pubmed/34222125 http://dx.doi.org/10.7774/cevr.2021.10.2.132 |
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author | Negm, Iman Ibrahim Ragab, Yasser M. Mohamed, Aly Fahmy |
author_facet | Negm, Iman Ibrahim Ragab, Yasser M. Mohamed, Aly Fahmy |
author_sort | Negm, Iman Ibrahim |
collection | PubMed |
description | PURPOSE: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). MATERIALS AND METHODS: Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total immunoglobulin G secreted interferon-gamma (IFN-γ), and the percentage of proliferated CD4+ and CD8+ T cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles. RESULTS: OMP adjuvanted rabies vaccine (FRV/K2+OMP) (OMP combined locally prepared vaccine) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) (alum adsorbed locally prepared vaccine). (FRV/K2+OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2+OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at p<0.05. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines. CONCLUSION: Outer membrane proteins adjuvanted rabies vaccines would be beneficial to induce rapid neutralizing antibodies and essential cytokines. |
format | Online Article Text |
id | pubmed-8217580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Vaccine Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-82175802021-07-01 Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine Negm, Iman Ibrahim Ragab, Yasser M. Mohamed, Aly Fahmy Clin Exp Vaccine Res Original Article PURPOSE: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). MATERIALS AND METHODS: Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total immunoglobulin G secreted interferon-gamma (IFN-γ), and the percentage of proliferated CD4+ and CD8+ T cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles. RESULTS: OMP adjuvanted rabies vaccine (FRV/K2+OMP) (OMP combined locally prepared vaccine) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) (alum adsorbed locally prepared vaccine). (FRV/K2+OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2+OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at p<0.05. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines. CONCLUSION: Outer membrane proteins adjuvanted rabies vaccines would be beneficial to induce rapid neutralizing antibodies and essential cytokines. The Korean Vaccine Society 2021-05 2021-05-31 /pmc/articles/PMC8217580/ /pubmed/34222125 http://dx.doi.org/10.7774/cevr.2021.10.2.132 Text en © Korean Vaccine Society. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Negm, Iman Ibrahim Ragab, Yasser M. Mohamed, Aly Fahmy Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine |
title | Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine |
title_full | Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine |
title_fullStr | Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine |
title_full_unstemmed | Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine |
title_short | Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine |
title_sort | outer membrane proteins of salmonella typhimurium as an adjuvant in rabies vaccine |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217580/ https://www.ncbi.nlm.nih.gov/pubmed/34222125 http://dx.doi.org/10.7774/cevr.2021.10.2.132 |
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