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Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine

PURPOSE: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). MATERIALS AND METHODS: Six groups of female Sprague Dawley albino rats (10/g...

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Autores principales: Negm, Iman Ibrahim, Ragab, Yasser M., Mohamed, Aly Fahmy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217580/
https://www.ncbi.nlm.nih.gov/pubmed/34222125
http://dx.doi.org/10.7774/cevr.2021.10.2.132
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author Negm, Iman Ibrahim
Ragab, Yasser M.
Mohamed, Aly Fahmy
author_facet Negm, Iman Ibrahim
Ragab, Yasser M.
Mohamed, Aly Fahmy
author_sort Negm, Iman Ibrahim
collection PubMed
description PURPOSE: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). MATERIALS AND METHODS: Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total immunoglobulin G secreted interferon-gamma (IFN-γ), and the percentage of proliferated CD4+ and CD8+ T cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles. RESULTS: OMP adjuvanted rabies vaccine (FRV/K2+OMP) (OMP combined locally prepared vaccine) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) (alum adsorbed locally prepared vaccine). (FRV/K2+OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2+OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at p<0.05. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines. CONCLUSION: Outer membrane proteins adjuvanted rabies vaccines would be beneficial to induce rapid neutralizing antibodies and essential cytokines.
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spelling pubmed-82175802021-07-01 Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine Negm, Iman Ibrahim Ragab, Yasser M. Mohamed, Aly Fahmy Clin Exp Vaccine Res Original Article PURPOSE: The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2). MATERIALS AND METHODS: Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total immunoglobulin G secreted interferon-gamma (IFN-γ), and the percentage of proliferated CD4+ and CD8+ T cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles. RESULTS: OMP adjuvanted rabies vaccine (FRV/K2+OMP) (OMP combined locally prepared vaccine) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) (alum adsorbed locally prepared vaccine). (FRV/K2+OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2+OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at p<0.05. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines. CONCLUSION: Outer membrane proteins adjuvanted rabies vaccines would be beneficial to induce rapid neutralizing antibodies and essential cytokines. The Korean Vaccine Society 2021-05 2021-05-31 /pmc/articles/PMC8217580/ /pubmed/34222125 http://dx.doi.org/10.7774/cevr.2021.10.2.132 Text en © Korean Vaccine Society. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Negm, Iman Ibrahim
Ragab, Yasser M.
Mohamed, Aly Fahmy
Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
title Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
title_full Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
title_fullStr Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
title_full_unstemmed Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
title_short Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
title_sort outer membrane proteins of salmonella typhimurium as an adjuvant in rabies vaccine
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217580/
https://www.ncbi.nlm.nih.gov/pubmed/34222125
http://dx.doi.org/10.7774/cevr.2021.10.2.132
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