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Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy

INTRODUCTION: Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progester...

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Autores principales: Arcos-Montoya, Denisse, Wegman-Ostrosky, Talia, Mejía-Pérez, Sonia, De la Fuente-Granada, Marisol, Camacho-Arroyo, Ignacio, García-Carrancá, Alejandro, Velasco-Velázquez, Marco A, Manjarrez-Marmolejo, Joaquín, González-Arenas, Aliesha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217595/
https://www.ncbi.nlm.nih.gov/pubmed/34168461
http://dx.doi.org/10.2147/OTT.S280314
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author Arcos-Montoya, Denisse
Wegman-Ostrosky, Talia
Mejía-Pérez, Sonia
De la Fuente-Granada, Marisol
Camacho-Arroyo, Ignacio
García-Carrancá, Alejandro
Velasco-Velázquez, Marco A
Manjarrez-Marmolejo, Joaquín
González-Arenas, Aliesha
author_facet Arcos-Montoya, Denisse
Wegman-Ostrosky, Talia
Mejía-Pérez, Sonia
De la Fuente-Granada, Marisol
Camacho-Arroyo, Ignacio
García-Carrancá, Alejandro
Velasco-Velázquez, Marco A
Manjarrez-Marmolejo, Joaquín
González-Arenas, Aliesha
author_sort Arcos-Montoya, Denisse
collection PubMed
description INTRODUCTION: Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progesterone receptor (PR), whose transcriptional activity could be regulated by phosphorylation by protein kinase C alpha (PKCα) at the residue Ser400. Our aim was to investigate if PR phosphorylation together with PKCα expression could be used as a prognostic factor for astrocytomas malignancy. METHODS: By immunofluorescence, we detected the content of PKCα, PR and its phosphorylation at Ser400 in 46 biopsies from Mexican patients with different astrocytoma malignancy grades; by bioinformatic tools using TCGA data, we evaluated the expression of PR and PKCα mRNA according to astrocytoma malignancy grades. For all statistical analyses, significance was p<0.05. RESULTS: We detected a positive correlation between the tumor grade and the content of PKCα, PR and its phosphorylation at Ser400, as well as the intracellular colocalization of these proteins. Interestingly, using an in silico assay, we found that the PR and PKCα expression at mRNA level has an inverse ratio with astrocytomas tumor grade. DISCUSSION: These results indicate that PR and its phosphorylation at Ser400 site, as well as PKCα and their colocalization, could be considered as possible malignancy biomarkers for astrocytomas grades I–IV.
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spelling pubmed-82175952021-06-23 Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy Arcos-Montoya, Denisse Wegman-Ostrosky, Talia Mejía-Pérez, Sonia De la Fuente-Granada, Marisol Camacho-Arroyo, Ignacio García-Carrancá, Alejandro Velasco-Velázquez, Marco A Manjarrez-Marmolejo, Joaquín González-Arenas, Aliesha Onco Targets Ther Original Research INTRODUCTION: Astrocytomas are the most common and aggressive primary brain tumors, and they are classified according to the degree of malignancy on a scale of I to IV, in which grade I is the least malignant and grade IV the highest. Many factors are related to astrocytomas progression as progesterone receptor (PR), whose transcriptional activity could be regulated by phosphorylation by protein kinase C alpha (PKCα) at the residue Ser400. Our aim was to investigate if PR phosphorylation together with PKCα expression could be used as a prognostic factor for astrocytomas malignancy. METHODS: By immunofluorescence, we detected the content of PKCα, PR and its phosphorylation at Ser400 in 46 biopsies from Mexican patients with different astrocytoma malignancy grades; by bioinformatic tools using TCGA data, we evaluated the expression of PR and PKCα mRNA according to astrocytoma malignancy grades. For all statistical analyses, significance was p<0.05. RESULTS: We detected a positive correlation between the tumor grade and the content of PKCα, PR and its phosphorylation at Ser400, as well as the intracellular colocalization of these proteins. Interestingly, using an in silico assay, we found that the PR and PKCα expression at mRNA level has an inverse ratio with astrocytomas tumor grade. DISCUSSION: These results indicate that PR and its phosphorylation at Ser400 site, as well as PKCα and their colocalization, could be considered as possible malignancy biomarkers for astrocytomas grades I–IV. Dove 2021-06-16 /pmc/articles/PMC8217595/ /pubmed/34168461 http://dx.doi.org/10.2147/OTT.S280314 Text en © 2021 Arcos-Montoya et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Arcos-Montoya, Denisse
Wegman-Ostrosky, Talia
Mejía-Pérez, Sonia
De la Fuente-Granada, Marisol
Camacho-Arroyo, Ignacio
García-Carrancá, Alejandro
Velasco-Velázquez, Marco A
Manjarrez-Marmolejo, Joaquín
González-Arenas, Aliesha
Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
title Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
title_full Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
title_fullStr Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
title_full_unstemmed Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
title_short Progesterone Receptor Together with PKCα Expression as Prognostic Factors for Astrocytomas Malignancy
title_sort progesterone receptor together with pkcα expression as prognostic factors for astrocytomas malignancy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8217595/
https://www.ncbi.nlm.nih.gov/pubmed/34168461
http://dx.doi.org/10.2147/OTT.S280314
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