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Light-Powered Reactivation of Flagella and Contraction of Microtubule Networks: Toward Building an Artificial Cell
[Image: see text] Artificial systems capable of self-sustained movement with self-sufficient energy are of high interest with respect to the development of many challenging applications, including medical treatments, but also technical applications. The bottom-up assembly of such systems in the cont...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218302/ https://www.ncbi.nlm.nih.gov/pubmed/33761235 http://dx.doi.org/10.1021/acssynbio.1c00071 |
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author | Ahmad, Raheel Kleineberg, Christin Nasirimarekani, Vahid Su, Yu-Jung Goli Pozveh, Samira Bae, Albert Sundmacher, Kai Bodenschatz, Eberhard Guido, Isabella Vidaković-koch, Tanja Gholami, Azam |
author_facet | Ahmad, Raheel Kleineberg, Christin Nasirimarekani, Vahid Su, Yu-Jung Goli Pozveh, Samira Bae, Albert Sundmacher, Kai Bodenschatz, Eberhard Guido, Isabella Vidaković-koch, Tanja Gholami, Azam |
author_sort | Ahmad, Raheel |
collection | PubMed |
description | [Image: see text] Artificial systems capable of self-sustained movement with self-sufficient energy are of high interest with respect to the development of many challenging applications, including medical treatments, but also technical applications. The bottom-up assembly of such systems in the context of synthetic biology is still a challenging task. In this work, we demonstrate the biocompatibility and efficiency of an artificial light-driven energy module and a motility functional unit by integrating light-switchable photosynthetic vesicles with demembranated flagella. The flagellar propulsion is coupled to the beating frequency, and dynamic ATP synthesis in response to illumination allows us to control beating frequency of flagella in a light-dependent manner. In addition, we verified the functionality of light-powered synthetic vesicles in in vitro motility assays by encapsulating microtubules assembled with force-generating kinesin-1 motors and the energy module to investigate the dynamics of a contractile filamentous network in cell-like compartments by optical stimulation. Integration of this photosynthetic system with various biological building blocks such as cytoskeletal filaments and molecular motors may contribute to the bottom-up synthesis of artificial cells that are able to undergo motor-driven morphological deformations and exhibit directional motion in a light-controllable fashion. |
format | Online Article Text |
id | pubmed-8218302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American
Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-82183022021-06-22 Light-Powered Reactivation of Flagella and Contraction of Microtubule Networks: Toward Building an Artificial Cell Ahmad, Raheel Kleineberg, Christin Nasirimarekani, Vahid Su, Yu-Jung Goli Pozveh, Samira Bae, Albert Sundmacher, Kai Bodenschatz, Eberhard Guido, Isabella Vidaković-koch, Tanja Gholami, Azam ACS Synth Biol [Image: see text] Artificial systems capable of self-sustained movement with self-sufficient energy are of high interest with respect to the development of many challenging applications, including medical treatments, but also technical applications. The bottom-up assembly of such systems in the context of synthetic biology is still a challenging task. In this work, we demonstrate the biocompatibility and efficiency of an artificial light-driven energy module and a motility functional unit by integrating light-switchable photosynthetic vesicles with demembranated flagella. The flagellar propulsion is coupled to the beating frequency, and dynamic ATP synthesis in response to illumination allows us to control beating frequency of flagella in a light-dependent manner. In addition, we verified the functionality of light-powered synthetic vesicles in in vitro motility assays by encapsulating microtubules assembled with force-generating kinesin-1 motors and the energy module to investigate the dynamics of a contractile filamentous network in cell-like compartments by optical stimulation. Integration of this photosynthetic system with various biological building blocks such as cytoskeletal filaments and molecular motors may contribute to the bottom-up synthesis of artificial cells that are able to undergo motor-driven morphological deformations and exhibit directional motion in a light-controllable fashion. American Chemical Society 2021-03-24 2021-06-18 /pmc/articles/PMC8218302/ /pubmed/33761235 http://dx.doi.org/10.1021/acssynbio.1c00071 Text en © 2021 The Authors. Published by American Chemical Society Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Ahmad, Raheel Kleineberg, Christin Nasirimarekani, Vahid Su, Yu-Jung Goli Pozveh, Samira Bae, Albert Sundmacher, Kai Bodenschatz, Eberhard Guido, Isabella Vidaković-koch, Tanja Gholami, Azam Light-Powered Reactivation of Flagella and Contraction of Microtubule Networks: Toward Building an Artificial Cell |
title | Light-Powered Reactivation of Flagella and Contraction
of Microtubule Networks: Toward Building an Artificial Cell |
title_full | Light-Powered Reactivation of Flagella and Contraction
of Microtubule Networks: Toward Building an Artificial Cell |
title_fullStr | Light-Powered Reactivation of Flagella and Contraction
of Microtubule Networks: Toward Building an Artificial Cell |
title_full_unstemmed | Light-Powered Reactivation of Flagella and Contraction
of Microtubule Networks: Toward Building an Artificial Cell |
title_short | Light-Powered Reactivation of Flagella and Contraction
of Microtubule Networks: Toward Building an Artificial Cell |
title_sort | light-powered reactivation of flagella and contraction
of microtubule networks: toward building an artificial cell |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218302/ https://www.ncbi.nlm.nih.gov/pubmed/33761235 http://dx.doi.org/10.1021/acssynbio.1c00071 |
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