Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri

KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that bla(KPC-82) is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nu...

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Autores principales: Lebreton, Francois, Corey, Brendan W., McElheny, Christi L., Iovleva, Alina, Preston, Lan, Margulieux, Katie R., Cybulski, Robert J., Mc Gann, Patrick, Doi, Yohei, Bennett, Jason W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Mechanisms of Resistance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218621/
https://www.ncbi.nlm.nih.gov/pubmed/33972237
http://dx.doi.org/10.1128/AAC.00150-21
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author Lebreton, Francois
Corey, Brendan W.
McElheny, Christi L.
Iovleva, Alina
Preston, Lan
Margulieux, Katie R.
Cybulski, Robert J.
Mc Gann, Patrick
Doi, Yohei
Bennett, Jason W.
author_facet Lebreton, Francois
Corey, Brendan W.
McElheny, Christi L.
Iovleva, Alina
Preston, Lan
Margulieux, Katie R.
Cybulski, Robert J.
Mc Gann, Patrick
Doi, Yohei
Bennett, Jason W.
author_sort Lebreton, Francois
collection PubMed
description KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that bla(KPC-82) is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala(267)-Ser(275) loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/β-lactamase inhibitor combinations.
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spelling pubmed-82186212021-12-17 Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri Lebreton, Francois Corey, Brendan W. McElheny, Christi L. Iovleva, Alina Preston, Lan Margulieux, Katie R. Cybulski, Robert J. Mc Gann, Patrick Doi, Yohei Bennett, Jason W. Antimicrob Agents Chemother Mechanisms of Resistance KPC-82 is a KPC-2 variant identified in a carbapenem-nonsusceptible Citrobacter koseri that confers high-level resistance to ceftazidime-avibactam. Genomic analysis revealed that bla(KPC-82) is carried by a chromosomally integrated Tn4401 transposon (disrupting porin gene phoE) and evolved by a 6-nucleotide tandem repeat duplication causing a two-amino-acid insertion (Ser-Asp) within the Ala(267)-Ser(275) loop. Similar to related KPC variants, KPC-82 showed decreased carbapenemase activity when expressed in a heterologous background and remained susceptible to carbapenem/β-lactamase inhibitor combinations. American Society for Microbiology 2021-06-17 /pmc/articles/PMC8218621/ /pubmed/33972237 http://dx.doi.org/10.1128/AAC.00150-21 Text en Copyright © 2021 Lebreton et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mechanisms of Resistance
Lebreton, Francois
Corey, Brendan W.
McElheny, Christi L.
Iovleva, Alina
Preston, Lan
Margulieux, Katie R.
Cybulski, Robert J.
Mc Gann, Patrick
Doi, Yohei
Bennett, Jason W.
Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri
title Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri
title_full Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri
title_fullStr Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri
title_full_unstemmed Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri
title_short Characterization of KPC-82, a KPC-2 Variant Conferring Resistance to Ceftazidime-Avibactam in a Carbapenem-Nonsusceptible Clinical Isolate of Citrobacter koseri
title_sort characterization of kpc-82, a kpc-2 variant conferring resistance to ceftazidime-avibactam in a carbapenem-nonsusceptible clinical isolate of citrobacter koseri
topic Mechanisms of Resistance
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218621/
https://www.ncbi.nlm.nih.gov/pubmed/33972237
http://dx.doi.org/10.1128/AAC.00150-21
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