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A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis
Seed germination is regulated by multiple phytohormones, including gibberellins (GAs) and brassinosteroids (BRs); however, the molecular mechanism underlying GA and BR co-induced seed germination is not well elucidated. We demonstrated that BRs induce seed germination through promoting testa and end...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219041/ https://www.ncbi.nlm.nih.gov/pubmed/33963401 http://dx.doi.org/10.1093/jxb/erab192 |
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author | Zhong, Chunmei Patra, Barunava Tang, Yi Li, Xukun Yuan, Ling Wang, Xiaojing |
author_facet | Zhong, Chunmei Patra, Barunava Tang, Yi Li, Xukun Yuan, Ling Wang, Xiaojing |
author_sort | Zhong, Chunmei |
collection | PubMed |
description | Seed germination is regulated by multiple phytohormones, including gibberellins (GAs) and brassinosteroids (BRs); however, the molecular mechanism underlying GA and BR co-induced seed germination is not well elucidated. We demonstrated that BRs induce seed germination through promoting testa and endosperm rupture in Arabidopsis. BRs promote cell elongation, rather than cell division, at the hypocotyl–radicle transition region of the embryonic axis during endosperm rupture. Two key basic helix–loop–helix transcription factors in the BR signaling pathway, HBI1 and BEE2, are involved in the regulation of endosperm rupture. Expression of HBI1 and BEE2 was induced in response to BR and GA treatment. In addition, HBI1- or BEE2-overexpressing Arabidopsis plants are less sensitive to the BR biosynthesis inhibitor, brassinazole, and the GA biosynthesis inhibitor, paclobutrazol. HBI1 and BEE2 promote endosperm rupture and seed germination by directly regulating the GA-Stimulated Arabidopsis 6 (GASA6) gene. Expression of GASA6 was altered in Arabidopsis overexpressing HBI1, BEE2, or SRDX-repressor forms of the two transcription factors. In addition, HBI1 interacts with BEE2 to synergistically activate GASA6 expression. Our findings define a new role for GASA6 in GA and BR signaling and reveal a regulatory module that controls GA and BR co-induced seed germination in Arabidopsis. |
format | Online Article Text |
id | pubmed-8219041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82190412021-06-23 A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis Zhong, Chunmei Patra, Barunava Tang, Yi Li, Xukun Yuan, Ling Wang, Xiaojing J Exp Bot Research Papers Seed germination is regulated by multiple phytohormones, including gibberellins (GAs) and brassinosteroids (BRs); however, the molecular mechanism underlying GA and BR co-induced seed germination is not well elucidated. We demonstrated that BRs induce seed germination through promoting testa and endosperm rupture in Arabidopsis. BRs promote cell elongation, rather than cell division, at the hypocotyl–radicle transition region of the embryonic axis during endosperm rupture. Two key basic helix–loop–helix transcription factors in the BR signaling pathway, HBI1 and BEE2, are involved in the regulation of endosperm rupture. Expression of HBI1 and BEE2 was induced in response to BR and GA treatment. In addition, HBI1- or BEE2-overexpressing Arabidopsis plants are less sensitive to the BR biosynthesis inhibitor, brassinazole, and the GA biosynthesis inhibitor, paclobutrazol. HBI1 and BEE2 promote endosperm rupture and seed germination by directly regulating the GA-Stimulated Arabidopsis 6 (GASA6) gene. Expression of GASA6 was altered in Arabidopsis overexpressing HBI1, BEE2, or SRDX-repressor forms of the two transcription factors. In addition, HBI1 interacts with BEE2 to synergistically activate GASA6 expression. Our findings define a new role for GASA6 in GA and BR signaling and reveal a regulatory module that controls GA and BR co-induced seed germination in Arabidopsis. Oxford University Press 2021-05-08 /pmc/articles/PMC8219041/ /pubmed/33963401 http://dx.doi.org/10.1093/jxb/erab192 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Zhong, Chunmei Patra, Barunava Tang, Yi Li, Xukun Yuan, Ling Wang, Xiaojing A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis |
title | A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis |
title_full | A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis |
title_fullStr | A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis |
title_full_unstemmed | A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis |
title_short | A transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in Arabidopsis |
title_sort | transcriptional hub integrating gibberellin–brassinosteroid signals to promote seed germination in arabidopsis |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219041/ https://www.ncbi.nlm.nih.gov/pubmed/33963401 http://dx.doi.org/10.1093/jxb/erab192 |
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