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Microscope-AOtools: a generalised adaptive optics implementation

Aberrations arising from sources such as sample heterogeneity and refractive index mismatches are constant problems in biological imaging. These aberrations reduce image quality and the achievable depth of imaging, particularly in super-resolution microscopy techniques. Adaptive optics (AO) technolo...

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Detalles Bibliográficos
Autores principales: Hall, Nicholas, Titlow, Josh, Booth, Martin J., Dobbie, Ian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219375/
https://www.ncbi.nlm.nih.gov/pubmed/33114806
http://dx.doi.org/10.1364/OE.401117
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author Hall, Nicholas
Titlow, Josh
Booth, Martin J.
Dobbie, Ian M.
author_facet Hall, Nicholas
Titlow, Josh
Booth, Martin J.
Dobbie, Ian M.
author_sort Hall, Nicholas
collection PubMed
description Aberrations arising from sources such as sample heterogeneity and refractive index mismatches are constant problems in biological imaging. These aberrations reduce image quality and the achievable depth of imaging, particularly in super-resolution microscopy techniques. Adaptive optics (AO) technology has been proven to be effective in correcting for these aberrations, thereby improving the image quality. However, it has not been widely adopted by the biological imaging community due, in part, to difficulty in set-up and operation of AO. The methods for doing so are not novel or unknown, but new users often waste time and effort reimplementing existing methods for their specific set-ups, hardware, sample types, etc. Microscope-AOtools offers a robust, easy-to-use implementation of the essential methods for set-up and use of AO elements and techniques. These methods are constructed in a generalised manner that can utilise a range of adaptive optics elements, wavefront sensing techniques and sensorless AO correction methods. Furthermore, the methods are designed to be easily extensible as new techniques arise, leading to a streamlined pipeline for new AO technology and techniques to be adopted by the wider microscopy community.
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spelling pubmed-82193752021-06-30 Microscope-AOtools: a generalised adaptive optics implementation Hall, Nicholas Titlow, Josh Booth, Martin J. Dobbie, Ian M. Opt Express Article Aberrations arising from sources such as sample heterogeneity and refractive index mismatches are constant problems in biological imaging. These aberrations reduce image quality and the achievable depth of imaging, particularly in super-resolution microscopy techniques. Adaptive optics (AO) technology has been proven to be effective in correcting for these aberrations, thereby improving the image quality. However, it has not been widely adopted by the biological imaging community due, in part, to difficulty in set-up and operation of AO. The methods for doing so are not novel or unknown, but new users often waste time and effort reimplementing existing methods for their specific set-ups, hardware, sample types, etc. Microscope-AOtools offers a robust, easy-to-use implementation of the essential methods for set-up and use of AO elements and techniques. These methods are constructed in a generalised manner that can utilise a range of adaptive optics elements, wavefront sensing techniques and sensorless AO correction methods. Furthermore, the methods are designed to be easily extensible as new techniques arise, leading to a streamlined pipeline for new AO technology and techniques to be adopted by the wider microscopy community. Optical Society of America 2020-09-15 /pmc/articles/PMC8219375/ /pubmed/33114806 http://dx.doi.org/10.1364/OE.401117 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. https://creativecommons.org/licenses/by/4.0/Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
spellingShingle Article
Hall, Nicholas
Titlow, Josh
Booth, Martin J.
Dobbie, Ian M.
Microscope-AOtools: a generalised adaptive optics implementation
title Microscope-AOtools: a generalised adaptive optics implementation
title_full Microscope-AOtools: a generalised adaptive optics implementation
title_fullStr Microscope-AOtools: a generalised adaptive optics implementation
title_full_unstemmed Microscope-AOtools: a generalised adaptive optics implementation
title_short Microscope-AOtools: a generalised adaptive optics implementation
title_sort microscope-aotools: a generalised adaptive optics implementation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219375/
https://www.ncbi.nlm.nih.gov/pubmed/33114806
http://dx.doi.org/10.1364/OE.401117
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