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Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages

The evolutionary “success” of the genus Brucella depends on the ability to persist both in the environment as well as inside of even activated macrophages of the animal host. For that, the Brucellae produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of t...

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Autores principales: Jacob, Jens, Finke, Antje, Mielke, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219583/
https://www.ncbi.nlm.nih.gov/pubmed/32462327
http://dx.doi.org/10.1007/s12223-020-00798-1
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author Jacob, Jens
Finke, Antje
Mielke, Martin
author_facet Jacob, Jens
Finke, Antje
Mielke, Martin
author_sort Jacob, Jens
collection PubMed
description The evolutionary “success” of the genus Brucella depends on the ability to persist both in the environment as well as inside of even activated macrophages of the animal host. For that, the Brucellae produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of the mglA gene in the B. abortus S19 vaccine strain resulted not only in an increased tolerance to H(2)O(2) but also in the induction of cytokines in macrophages, we here investigated the effect of oxidative stress (Fe(2+) and H(2)O(2)) on the survival of B. abortus S19 and the isogenic B. abortus S 19 ∆mglA 3.14 deletion mutant in comparison with B. neotomae 5K33, Brucella strain 83/13, and B. microti CCM4915. These Brucellae belong to different phylogenetic clades and show characteristic differences in the mgl-operon. From the various Brucellae tested, B. abortus S19 showed the highest susceptibility to oxidative stress and the lowest ability to survive inside of murine macrophages. B. abortus S19 ∆mglA 3.14 as well as B. neotomae, which also belongs to the classical core clade of Brucella and lacks the regulators of the mgl-operon, presented the highest degree of tolerance to H(2)O(2) but not in the survival in macrophages. The latter was most pronounced in case of an infection with B. 83/13 and B. microti CCM4915. The various Brucellae investigated here demonstrate significant differences in tolerance against oxidative stress and different survival in murine macrophages, which, however, do not correlate directly.
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spelling pubmed-82195832021-06-28 Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages Jacob, Jens Finke, Antje Mielke, Martin Folia Microbiol (Praha) Original Article The evolutionary “success” of the genus Brucella depends on the ability to persist both in the environment as well as inside of even activated macrophages of the animal host. For that, the Brucellae produce catalase and superoxide dismutase to defend against oxidative stress. Since the deletion of the mglA gene in the B. abortus S19 vaccine strain resulted not only in an increased tolerance to H(2)O(2) but also in the induction of cytokines in macrophages, we here investigated the effect of oxidative stress (Fe(2+) and H(2)O(2)) on the survival of B. abortus S19 and the isogenic B. abortus S 19 ∆mglA 3.14 deletion mutant in comparison with B. neotomae 5K33, Brucella strain 83/13, and B. microti CCM4915. These Brucellae belong to different phylogenetic clades and show characteristic differences in the mgl-operon. From the various Brucellae tested, B. abortus S19 showed the highest susceptibility to oxidative stress and the lowest ability to survive inside of murine macrophages. B. abortus S19 ∆mglA 3.14 as well as B. neotomae, which also belongs to the classical core clade of Brucella and lacks the regulators of the mgl-operon, presented the highest degree of tolerance to H(2)O(2) but not in the survival in macrophages. The latter was most pronounced in case of an infection with B. 83/13 and B. microti CCM4915. The various Brucellae investigated here demonstrate significant differences in tolerance against oxidative stress and different survival in murine macrophages, which, however, do not correlate directly. Springer Netherlands 2020-05-28 2020 /pmc/articles/PMC8219583/ /pubmed/32462327 http://dx.doi.org/10.1007/s12223-020-00798-1 Text en © The Author(s) 2020, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Jacob, Jens
Finke, Antje
Mielke, Martin
Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
title Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
title_full Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
title_fullStr Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
title_full_unstemmed Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
title_short Survival of Brucella abortus S19 and other Brucella spp. in the presence of oxidative stress and within macrophages
title_sort survival of brucella abortus s19 and other brucella spp. in the presence of oxidative stress and within macrophages
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219583/
https://www.ncbi.nlm.nih.gov/pubmed/32462327
http://dx.doi.org/10.1007/s12223-020-00798-1
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