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Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans
OBJECTIVE: The aim of this study was to evaluate the effect of nystatin on the efficacy of chlorhexidine against Streptococcus mutans in planktonic cells and mixed biofilm with Candida albicans. MATERIAL AND METHODS: S. mutans ATCC 25,175 in suspension and also combined with C. albicans ATCC 18,804...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219785/ https://www.ncbi.nlm.nih.gov/pubmed/34160701 http://dx.doi.org/10.1007/s00784-021-04041-0 |
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author | Baldino, Maria Eduarda Lanes Medina-Silva, Renata Sumienski, Juliana Figueiredo, Maria Antonia Salum, Fernanda Gonçalves Cherubini, Karen |
author_facet | Baldino, Maria Eduarda Lanes Medina-Silva, Renata Sumienski, Juliana Figueiredo, Maria Antonia Salum, Fernanda Gonçalves Cherubini, Karen |
author_sort | Baldino, Maria Eduarda Lanes |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to evaluate the effect of nystatin on the efficacy of chlorhexidine against Streptococcus mutans in planktonic cells and mixed biofilm with Candida albicans. MATERIAL AND METHODS: S. mutans ATCC 25,175 in suspension and also combined with C. albicans ATCC 18,804 in biofilm were cultured. Minimum inhibitory concentration (MIC), crystal violet colorimetric assay, and colony-forming unit counting (CFUs/mL) were performed. RESULTS: An increased MIC of chlorhexidine against S. mutans was observed when the drugs were administered mixed in a single formulation and with time intervals in between, except for the 30-min interval. The biofilm optical density (OD) in treatments using chlorhexidine and nystatin combined did not significantly differ from chlorhexidine alone. Either in biofilm colorimetric assay or determination of CFUs, the combined treatments with nystatin administered before chlorhexidine had less effect on chlorhexidine efficacy. CONCLUSIONS: Nystatin interferes with the action of chlorhexidine against S. mutans. The antimicrobial effectiveness of the combined drugs depends on their concentration, time interval used, and the planktonic or biofilm behavior of the microorganisms. CLINICAL RELEVANCE: In view of the great number of patients that can receive a prescription of chlorhexidine and nystatin concomitantly, this study contributes to the knowledge about the effect of the combined drugs. Given the high prevalence of prescriptions of chlorhexidine and nystatin in dentistry, dental professionals should be aware of their possible antagonistic effect. |
format | Online Article Text |
id | pubmed-8219785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-82197852021-06-23 Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans Baldino, Maria Eduarda Lanes Medina-Silva, Renata Sumienski, Juliana Figueiredo, Maria Antonia Salum, Fernanda Gonçalves Cherubini, Karen Clin Oral Investig Original Article OBJECTIVE: The aim of this study was to evaluate the effect of nystatin on the efficacy of chlorhexidine against Streptococcus mutans in planktonic cells and mixed biofilm with Candida albicans. MATERIAL AND METHODS: S. mutans ATCC 25,175 in suspension and also combined with C. albicans ATCC 18,804 in biofilm were cultured. Minimum inhibitory concentration (MIC), crystal violet colorimetric assay, and colony-forming unit counting (CFUs/mL) were performed. RESULTS: An increased MIC of chlorhexidine against S. mutans was observed when the drugs were administered mixed in a single formulation and with time intervals in between, except for the 30-min interval. The biofilm optical density (OD) in treatments using chlorhexidine and nystatin combined did not significantly differ from chlorhexidine alone. Either in biofilm colorimetric assay or determination of CFUs, the combined treatments with nystatin administered before chlorhexidine had less effect on chlorhexidine efficacy. CONCLUSIONS: Nystatin interferes with the action of chlorhexidine against S. mutans. The antimicrobial effectiveness of the combined drugs depends on their concentration, time interval used, and the planktonic or biofilm behavior of the microorganisms. CLINICAL RELEVANCE: In view of the great number of patients that can receive a prescription of chlorhexidine and nystatin concomitantly, this study contributes to the knowledge about the effect of the combined drugs. Given the high prevalence of prescriptions of chlorhexidine and nystatin in dentistry, dental professionals should be aware of their possible antagonistic effect. Springer Berlin Heidelberg 2021-06-23 2022 /pmc/articles/PMC8219785/ /pubmed/34160701 http://dx.doi.org/10.1007/s00784-021-04041-0 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Baldino, Maria Eduarda Lanes Medina-Silva, Renata Sumienski, Juliana Figueiredo, Maria Antonia Salum, Fernanda Gonçalves Cherubini, Karen Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans |
title | Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans |
title_full | Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans |
title_fullStr | Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans |
title_full_unstemmed | Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans |
title_short | Nystatin effect on chlorhexidine efficacy against Streptococcus mutans as planktonic cells and mixed biofilm with Candida albicans |
title_sort | nystatin effect on chlorhexidine efficacy against streptococcus mutans as planktonic cells and mixed biofilm with candida albicans |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219785/ https://www.ncbi.nlm.nih.gov/pubmed/34160701 http://dx.doi.org/10.1007/s00784-021-04041-0 |
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