Cargando…
RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219824/ https://www.ncbi.nlm.nih.gov/pubmed/34158505 http://dx.doi.org/10.1038/s41467-021-24195-5 |
_version_ | 1783711020086198272 |
---|---|
author | Zhang, Tao Mudgett, Michael Rambabu, Ratnala Abramson, Bradley Dai, Xinhua Michael, Todd P. Zhao, Yunde |
author_facet | Zhang, Tao Mudgett, Michael Rambabu, Ratnala Abramson, Bradley Dai, Xinhua Michael, Todd P. Zhao, Yunde |
author_sort | Zhang, Tao |
collection | PubMed |
description | Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-based gene drives can generate biased transmission of a preferred allele and convert heterozygotes to homozygotes in insects and mice, but similar strategies have not been implementable in plants because of a lack of efficient homology-directed repair (HDR). Here, we place a gene drive, which consists of cassettes that produce Cas9, guide RNAs (gRNA), and fluorescent markers, into the CRYPTOCHROME 1 (CRY1) gene through CRISPR/Cas9-mediated HDR, resulting in cry1(drive) lines. After crossing the cry1(drive)/cry1(drive) lines to wild type, we observe F1 plants which have DNA at the CRY1 locus from only the cry1(drive)/cry1(drive) parent. Moreover, a non-autonomous trans-acting gene drive, in which the gene drive unit and the target gene are located on different chromosomes, converts a heterozygous mutation in the target gene to homozygous. Our results demonstrate that homozygous F1 plants can be obtained through zygotic conversion using a CRISPR/Cas9-based gene drive. |
format | Online Article Text |
id | pubmed-8219824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82198242021-07-09 RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis Zhang, Tao Mudgett, Michael Rambabu, Ratnala Abramson, Bradley Dai, Xinhua Michael, Todd P. Zhao, Yunde Nat Commun Article Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-based gene drives can generate biased transmission of a preferred allele and convert heterozygotes to homozygotes in insects and mice, but similar strategies have not been implementable in plants because of a lack of efficient homology-directed repair (HDR). Here, we place a gene drive, which consists of cassettes that produce Cas9, guide RNAs (gRNA), and fluorescent markers, into the CRYPTOCHROME 1 (CRY1) gene through CRISPR/Cas9-mediated HDR, resulting in cry1(drive) lines. After crossing the cry1(drive)/cry1(drive) lines to wild type, we observe F1 plants which have DNA at the CRY1 locus from only the cry1(drive)/cry1(drive) parent. Moreover, a non-autonomous trans-acting gene drive, in which the gene drive unit and the target gene are located on different chromosomes, converts a heterozygous mutation in the target gene to homozygous. Our results demonstrate that homozygous F1 plants can be obtained through zygotic conversion using a CRISPR/Cas9-based gene drive. Nature Publishing Group UK 2021-06-22 /pmc/articles/PMC8219824/ /pubmed/34158505 http://dx.doi.org/10.1038/s41467-021-24195-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Tao Mudgett, Michael Rambabu, Ratnala Abramson, Bradley Dai, Xinhua Michael, Todd P. Zhao, Yunde RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis |
title | RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis |
title_full | RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis |
title_fullStr | RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis |
title_full_unstemmed | RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis |
title_short | RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis |
title_sort | retracted article: selective inheritance of target genes from only one parent of sexually reproduced f1 progeny in arabidopsis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219824/ https://www.ncbi.nlm.nih.gov/pubmed/34158505 http://dx.doi.org/10.1038/s41467-021-24195-5 |
work_keys_str_mv | AT zhangtao retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis AT mudgettmichael retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis AT rambaburatnala retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis AT abramsonbradley retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis AT daixinhua retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis AT michaeltoddp retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis AT zhaoyunde retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis |