Cargando…

RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis

Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Tao, Mudgett, Michael, Rambabu, Ratnala, Abramson, Bradley, Dai, Xinhua, Michael, Todd P., Zhao, Yunde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219824/
https://www.ncbi.nlm.nih.gov/pubmed/34158505
http://dx.doi.org/10.1038/s41467-021-24195-5
_version_ 1783711020086198272
author Zhang, Tao
Mudgett, Michael
Rambabu, Ratnala
Abramson, Bradley
Dai, Xinhua
Michael, Todd P.
Zhao, Yunde
author_facet Zhang, Tao
Mudgett, Michael
Rambabu, Ratnala
Abramson, Bradley
Dai, Xinhua
Michael, Todd P.
Zhao, Yunde
author_sort Zhang, Tao
collection PubMed
description Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-based gene drives can generate biased transmission of a preferred allele and convert heterozygotes to homozygotes in insects and mice, but similar strategies have not been implementable in plants because of a lack of efficient homology-directed repair (HDR). Here, we place a gene drive, which consists of cassettes that produce Cas9, guide RNAs (gRNA), and fluorescent markers, into the CRYPTOCHROME 1 (CRY1) gene through CRISPR/Cas9-mediated HDR, resulting in cry1(drive) lines. After crossing the cry1(drive)/cry1(drive) lines to wild type, we observe F1 plants which have DNA at the CRY1 locus from only the cry1(drive)/cry1(drive) parent. Moreover, a non-autonomous trans-acting gene drive, in which the gene drive unit and the target gene are located on different chromosomes, converts a heterozygous mutation in the target gene to homozygous. Our results demonstrate that homozygous F1 plants can be obtained through zygotic conversion using a CRISPR/Cas9-based gene drive.
format Online
Article
Text
id pubmed-8219824
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-82198242021-07-09 RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis Zhang, Tao Mudgett, Michael Rambabu, Ratnala Abramson, Bradley Dai, Xinhua Michael, Todd P. Zhao, Yunde Nat Commun Article Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-based gene drives can generate biased transmission of a preferred allele and convert heterozygotes to homozygotes in insects and mice, but similar strategies have not been implementable in plants because of a lack of efficient homology-directed repair (HDR). Here, we place a gene drive, which consists of cassettes that produce Cas9, guide RNAs (gRNA), and fluorescent markers, into the CRYPTOCHROME 1 (CRY1) gene through CRISPR/Cas9-mediated HDR, resulting in cry1(drive) lines. After crossing the cry1(drive)/cry1(drive) lines to wild type, we observe F1 plants which have DNA at the CRY1 locus from only the cry1(drive)/cry1(drive) parent. Moreover, a non-autonomous trans-acting gene drive, in which the gene drive unit and the target gene are located on different chromosomes, converts a heterozygous mutation in the target gene to homozygous. Our results demonstrate that homozygous F1 plants can be obtained through zygotic conversion using a CRISPR/Cas9-based gene drive. Nature Publishing Group UK 2021-06-22 /pmc/articles/PMC8219824/ /pubmed/34158505 http://dx.doi.org/10.1038/s41467-021-24195-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhang, Tao
Mudgett, Michael
Rambabu, Ratnala
Abramson, Bradley
Dai, Xinhua
Michael, Todd P.
Zhao, Yunde
RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
title RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
title_full RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
title_fullStr RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
title_full_unstemmed RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
title_short RETRACTED ARTICLE: Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis
title_sort retracted article: selective inheritance of target genes from only one parent of sexually reproduced f1 progeny in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219824/
https://www.ncbi.nlm.nih.gov/pubmed/34158505
http://dx.doi.org/10.1038/s41467-021-24195-5
work_keys_str_mv AT zhangtao retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis
AT mudgettmichael retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis
AT rambaburatnala retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis
AT abramsonbradley retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis
AT daixinhua retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis
AT michaeltoddp retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis
AT zhaoyunde retractedarticleselectiveinheritanceoftargetgenesfromonlyoneparentofsexuallyreproducedf1progenyinarabidopsis