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Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle

Blood samples from 260 unrelated cattle (132 animals affected by papillomavirus-associated bladder tumors and 128 healthy) were genotyped using the classic polymerase chain reaction/restriction fragment length polymorphism method to screen MHC class II bovine leukocyte antigen-DRB3. 2 polymorphism....

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Autores principales: Longeri, Maria, Russo, Valeria, Strillacci, Maria Giuseppina, Perillo, Antonella, Carisetti, Michela, Cozzi, Maria Cristina, Neola, Benedetto, Roperto, Sante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219868/
https://www.ncbi.nlm.nih.gov/pubmed/34179154
http://dx.doi.org/10.3389/fvets.2021.630089
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author Longeri, Maria
Russo, Valeria
Strillacci, Maria Giuseppina
Perillo, Antonella
Carisetti, Michela
Cozzi, Maria Cristina
Neola, Benedetto
Roperto, Sante
author_facet Longeri, Maria
Russo, Valeria
Strillacci, Maria Giuseppina
Perillo, Antonella
Carisetti, Michela
Cozzi, Maria Cristina
Neola, Benedetto
Roperto, Sante
author_sort Longeri, Maria
collection PubMed
description Blood samples from 260 unrelated cattle (132 animals affected by papillomavirus-associated bladder tumors and 128 healthy) were genotyped using the classic polymerase chain reaction/restriction fragment length polymorphism method to screen MHC class II bovine leukocyte antigen-DRB3. 2 polymorphism. The DRB3(*)22 allele was significantly (p ≤ 0.01) detected in healthy cattle, thus appearing to have a negative association (protective effect) with virus infection of the urinary bladder known to represent a bladder tumor risk for cattle living free at pasture. Considering the two sequence alleles identified in animals carrying DRB3(*)22, DRB3(*)011:01 allele from samples of animals harboring the unexpressed bovine papillomaviruses (BPV)-2 E5 gene was characterized by amino acid residues believed to have a protective effect against BPV infection such as arginine at position 71 (R(71)) in pocket 4, histidine at position 11 (H(11)) in pocket 6, and both glutamine at position 9 (Q(9)) and serine at position 57 (S(57)) in pocket 9 of the antigen-binding groove. The DRB3(*)011:02v allele from affected animals was characterized by amino acids believed to be susceptibility residues such as lysine (K(71)), tyrosine (Y(11)), glutamic acid (E(9)), and aspartic acid (D(57)) in these pockets. These results suggest that animals harboring the DRB3(*)011:01 allele may have a lower risk of BPV infection and, consequently, a reduced risk of bladder tumors.
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spelling pubmed-82198682021-06-24 Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle Longeri, Maria Russo, Valeria Strillacci, Maria Giuseppina Perillo, Antonella Carisetti, Michela Cozzi, Maria Cristina Neola, Benedetto Roperto, Sante Front Vet Sci Veterinary Science Blood samples from 260 unrelated cattle (132 animals affected by papillomavirus-associated bladder tumors and 128 healthy) were genotyped using the classic polymerase chain reaction/restriction fragment length polymorphism method to screen MHC class II bovine leukocyte antigen-DRB3. 2 polymorphism. The DRB3(*)22 allele was significantly (p ≤ 0.01) detected in healthy cattle, thus appearing to have a negative association (protective effect) with virus infection of the urinary bladder known to represent a bladder tumor risk for cattle living free at pasture. Considering the two sequence alleles identified in animals carrying DRB3(*)22, DRB3(*)011:01 allele from samples of animals harboring the unexpressed bovine papillomaviruses (BPV)-2 E5 gene was characterized by amino acid residues believed to have a protective effect against BPV infection such as arginine at position 71 (R(71)) in pocket 4, histidine at position 11 (H(11)) in pocket 6, and both glutamine at position 9 (Q(9)) and serine at position 57 (S(57)) in pocket 9 of the antigen-binding groove. The DRB3(*)011:02v allele from affected animals was characterized by amino acids believed to be susceptibility residues such as lysine (K(71)), tyrosine (Y(11)), glutamic acid (E(9)), and aspartic acid (D(57)) in these pockets. These results suggest that animals harboring the DRB3(*)011:01 allele may have a lower risk of BPV infection and, consequently, a reduced risk of bladder tumors. Frontiers Media S.A. 2021-06-09 /pmc/articles/PMC8219868/ /pubmed/34179154 http://dx.doi.org/10.3389/fvets.2021.630089 Text en Copyright © 2021 Longeri, Russo, Strillacci, Perillo, Carisetti, Cozzi, Neola and Roperto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Longeri, Maria
Russo, Valeria
Strillacci, Maria Giuseppina
Perillo, Antonella
Carisetti, Michela
Cozzi, Maria Cristina
Neola, Benedetto
Roperto, Sante
Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
title Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
title_full Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
title_fullStr Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
title_full_unstemmed Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
title_short Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
title_sort association between bola-drb3.2 polymorphism and bovine papillomavirus infection for bladder tumor risk in podolica cattle
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8219868/
https://www.ncbi.nlm.nih.gov/pubmed/34179154
http://dx.doi.org/10.3389/fvets.2021.630089
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