Genome-wide mapping of DNA double-strand breaks from eukaryotic cell cultures using Break-seq

We describe a genome-wide DNA double-strand break (DSB) mapping technique, Break-seq. In this protocol, we provide step-by-step instructions for cell embedment in agarose, in-gel DSB labeling and subsequent capture, followed by standard Illumina library construction and sequencing. We also provide t...

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Detalles Bibliográficos
Autores principales: Joshi, Ishita, DeRycke, Jenna, Palmowski, Megan, LeSuer, Robert, Feng, Wenyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220103/
https://www.ncbi.nlm.nih.gov/pubmed/34189468
http://dx.doi.org/10.1016/j.xpro.2021.100554

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