Cargando…

Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry

Lumbar disc herniation (LDH) possesses complex pathogenesis, which has not been well elucidated yet. To date, specific or early diagnosis of LDH remains unavailable, resulting in missed opportunity for effective treatment. According to Traditional Chinese medicine (TCM) theory, LDH can be divided in...

Descripción completa

Detalles Bibliográficos
Autores principales: Shan, Letian, Yang, Jinying, Meng, Shijie, Ruan, Hongfeng, Zhou, Li, Ye, Fusheng, Tong, Peijian, Wu, Chengliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220151/
https://www.ncbi.nlm.nih.gov/pubmed/34179074
http://dx.doi.org/10.3389/fmolb.2021.648823
_version_ 1783711086746271744
author Shan, Letian
Yang, Jinying
Meng, Shijie
Ruan, Hongfeng
Zhou, Li
Ye, Fusheng
Tong, Peijian
Wu, Chengliang
author_facet Shan, Letian
Yang, Jinying
Meng, Shijie
Ruan, Hongfeng
Zhou, Li
Ye, Fusheng
Tong, Peijian
Wu, Chengliang
author_sort Shan, Letian
collection PubMed
description Lumbar disc herniation (LDH) possesses complex pathogenesis, which has not been well elucidated yet. To date, specific or early diagnosis of LDH remains unavailable, resulting in missed opportunity for effective treatment. According to Traditional Chinese medicine (TCM) theory, LDH can be divided into two subtypes (reality syndrome and deficiency syndrome). The purpose of this study was to analyze the metabolic disorders of LDH and its TCM subtypes and screen out potential biomarkers for LDH diagnosis. Gas chromatography coupled with mass spectrometry (GC-MS) was applied to test the urine samples from 66 participants (30 healthy volunteers, 18 LDH patients with deficiency syndrome and 18 patients with reality syndrome). PCA analysis showed a distinct separation tendency between the healthy subjects and LDH patients but no obvious separation between the different syndromes (reality syndrome and deficiency syndrome) of LDH patients. As a result, 23 metabolites were identified significantly altered in the LDH patients, as compared with the healthy subjects. The altered metabolites belong to amino acid metabolism, nucleic acid metabolism, carbohydrate metabolism, and vitamin metabolism, which are related to osteoporosis and inflammation. Our results indicate metabolic disorders of LDH and thereby propose a group of metabolic biomarkers for potential application in early diagnosis of LDH in clinic, which provide a reasonable explanation for the pathogenesis of LDH.
format Online
Article
Text
id pubmed-8220151
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82201512021-06-24 Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry Shan, Letian Yang, Jinying Meng, Shijie Ruan, Hongfeng Zhou, Li Ye, Fusheng Tong, Peijian Wu, Chengliang Front Mol Biosci Molecular Biosciences Lumbar disc herniation (LDH) possesses complex pathogenesis, which has not been well elucidated yet. To date, specific or early diagnosis of LDH remains unavailable, resulting in missed opportunity for effective treatment. According to Traditional Chinese medicine (TCM) theory, LDH can be divided into two subtypes (reality syndrome and deficiency syndrome). The purpose of this study was to analyze the metabolic disorders of LDH and its TCM subtypes and screen out potential biomarkers for LDH diagnosis. Gas chromatography coupled with mass spectrometry (GC-MS) was applied to test the urine samples from 66 participants (30 healthy volunteers, 18 LDH patients with deficiency syndrome and 18 patients with reality syndrome). PCA analysis showed a distinct separation tendency between the healthy subjects and LDH patients but no obvious separation between the different syndromes (reality syndrome and deficiency syndrome) of LDH patients. As a result, 23 metabolites were identified significantly altered in the LDH patients, as compared with the healthy subjects. The altered metabolites belong to amino acid metabolism, nucleic acid metabolism, carbohydrate metabolism, and vitamin metabolism, which are related to osteoporosis and inflammation. Our results indicate metabolic disorders of LDH and thereby propose a group of metabolic biomarkers for potential application in early diagnosis of LDH in clinic, which provide a reasonable explanation for the pathogenesis of LDH. Frontiers Media S.A. 2021-06-09 /pmc/articles/PMC8220151/ /pubmed/34179074 http://dx.doi.org/10.3389/fmolb.2021.648823 Text en Copyright © 2021 Shan, Yang, Meng, Ruan, Zhou, Ye, Tong and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Shan, Letian
Yang, Jinying
Meng, Shijie
Ruan, Hongfeng
Zhou, Li
Ye, Fusheng
Tong, Peijian
Wu, Chengliang
Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry
title Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry
title_full Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry
title_fullStr Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry
title_full_unstemmed Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry
title_short Urine Metabolomics Profiling of Lumbar Disc Herniation and its Traditional Chinese Medicine Subtypes in Patients Through Gas Chromatography Coupled With Mass Spectrometry
title_sort urine metabolomics profiling of lumbar disc herniation and its traditional chinese medicine subtypes in patients through gas chromatography coupled with mass spectrometry
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220151/
https://www.ncbi.nlm.nih.gov/pubmed/34179074
http://dx.doi.org/10.3389/fmolb.2021.648823
work_keys_str_mv AT shanletian urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT yangjinying urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT mengshijie urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT ruanhongfeng urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT zhouli urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT yefusheng urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT tongpeijian urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry
AT wuchengliang urinemetabolomicsprofilingoflumbardischerniationanditstraditionalchinesemedicinesubtypesinpatientsthroughgaschromatographycoupledwithmassspectrometry