Gut microbiota transplantation for colonization of germ-free mice

The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor m...

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Detalles Bibliográficos
Autores principales: Choo, Jocelyn M., Rogers, Geraint B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220245/
https://www.ncbi.nlm.nih.gov/pubmed/34189475
http://dx.doi.org/10.1016/j.xpro.2021.100610
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author Choo, Jocelyn M.
Rogers, Geraint B.
author_facet Choo, Jocelyn M.
Rogers, Geraint B.
author_sort Choo, Jocelyn M.
collection PubMed
description The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery. For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021).
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spelling pubmed-82202452021-06-28 Gut microbiota transplantation for colonization of germ-free mice Choo, Jocelyn M. Rogers, Geraint B. STAR Protoc Protocol The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery. For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021). Elsevier 2021-06-16 /pmc/articles/PMC8220245/ /pubmed/34189475 http://dx.doi.org/10.1016/j.xpro.2021.100610 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Choo, Jocelyn M.
Rogers, Geraint B.
Gut microbiota transplantation for colonization of germ-free mice
title Gut microbiota transplantation for colonization of germ-free mice
title_full Gut microbiota transplantation for colonization of germ-free mice
title_fullStr Gut microbiota transplantation for colonization of germ-free mice
title_full_unstemmed Gut microbiota transplantation for colonization of germ-free mice
title_short Gut microbiota transplantation for colonization of germ-free mice
title_sort gut microbiota transplantation for colonization of germ-free mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220245/
https://www.ncbi.nlm.nih.gov/pubmed/34189475
http://dx.doi.org/10.1016/j.xpro.2021.100610
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