A draft reference assembly of the Psilocybe cubensis genome

We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster...

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Detalles Bibliográficos
Autores principales: McKernan, Kevin, Kane, Liam T., Crawford, Seth, Chin, Chen-Shan, Trippe, Aaron, McLaughlin, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2021
Materias:
Data Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220353/
https://www.ncbi.nlm.nih.gov/pubmed/34322225
http://dx.doi.org/10.12688/f1000research.51613.2
Descripción
Sumario:We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.

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