A draft reference assembly of the Psilocybe cubensis genome

We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster...

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Detalles Bibliográficos
Autores principales: McKernan, Kevin, Kane, Liam T., Crawford, Seth, Chin, Chen-Shan, Trippe, Aaron, McLaughlin, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2021
Materias:
Data Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220353/
https://www.ncbi.nlm.nih.gov/pubmed/34322225
http://dx.doi.org/10.12688/f1000research.51613.2
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author McKernan, Kevin
Kane, Liam T.
Crawford, Seth
Chin, Chen-Shan
Trippe, Aaron
McLaughlin, Stephen
author_facet McKernan, Kevin
Kane, Liam T.
Crawford, Seth
Chin, Chen-Shan
Trippe, Aaron
McLaughlin, Stephen
author_sort McKernan, Kevin
collection PubMed
description We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.
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spelling pubmed-82203532021-07-27 A draft reference assembly of the Psilocybe cubensis genome McKernan, Kevin Kane, Liam T. Crawford, Seth Chin, Chen-Shan Trippe, Aaron McLaughlin, Stephen F1000Res Data Note We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437. F1000 Research Limited 2021-06-15 /pmc/articles/PMC8220353/ /pubmed/34322225 http://dx.doi.org/10.12688/f1000research.51613.2 Text en Copyright: © 2021 McKernan K et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Note
McKernan, Kevin
Kane, Liam T.
Crawford, Seth
Chin, Chen-Shan
Trippe, Aaron
McLaughlin, Stephen
A draft reference assembly of the Psilocybe cubensis genome
title A draft reference assembly of the Psilocybe cubensis genome
title_full A draft reference assembly of the Psilocybe cubensis genome
title_fullStr A draft reference assembly of the Psilocybe cubensis genome
title_full_unstemmed A draft reference assembly of the Psilocybe cubensis genome
title_short A draft reference assembly of the Psilocybe cubensis genome
title_sort draft reference assembly of the psilocybe cubensis genome
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220353/
https://www.ncbi.nlm.nih.gov/pubmed/34322225
http://dx.doi.org/10.12688/f1000research.51613.2
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