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Optimized protocol for tRNA identification in the ribosomal complexes from human cell lines

Here, we describe a protocol for tRNA identification in the 60S ribosome-nascent peptide complex co-purified with Nuclear Export Mediator Factor (NEMF), a responsible factor for C-terminal alanine and threonine tailing of the nascent peptide. Our protocol is based on regular reverse transcription fo...

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Detalles Bibliográficos
Autores principales:	Udagawa, Tsuyoshi, Seki, Moeka, Inada, Toshifumi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220392/ https://www.ncbi.nlm.nih.gov/pubmed/34189478 http://dx.doi.org/10.1016/j.xpro.2021.100615

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