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Isolation of nuclei from mouse white adipose tissues for single-nucleus genomics

Lipid-filled adipocytes are incompatible with droplet-based single-cell methods, such as 10x Genomics-based technology, thus restricting droplet-based single-cell analyses of adipose tissues to the stromal vascular fraction. To overcome this limitation and obtain cellular and molecular insight into...

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Detalles Bibliográficos
Autores principales:	Van Hauwaert, Elvira Laila, Gammelmark, Ellen, Sárvári, Anitta Kinga, Larsen, Lena, Nielsen, Ronni, Madsen, Jesper Grud Skat, Mandrup, Susanne
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220393/ https://www.ncbi.nlm.nih.gov/pubmed/34189477 http://dx.doi.org/10.1016/j.xpro.2021.100612

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