Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer

(13)C nuclear spin hyperpolarization can increase the sensitivity of detection in an MRI experiment by more than 10,000-fold. (13)C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized (13)C label exchange between injected [1-(13)C]pyruvate and the endogenous tumor lactate pool can be u...

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Detalles Bibliográficos
Autores principales: Ros, Susana, Wright, Alan J., Bruna, Alejandra, Caldas, Carlos, Brindle, Kevin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220404/
https://www.ncbi.nlm.nih.gov/pubmed/34189473
http://dx.doi.org/10.1016/j.xpro.2021.100608
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author Ros, Susana
Wright, Alan J.
Bruna, Alejandra
Caldas, Carlos
Brindle, Kevin M.
author_facet Ros, Susana
Wright, Alan J.
Bruna, Alejandra
Caldas, Carlos
Brindle, Kevin M.
author_sort Ros, Susana
collection PubMed
description (13)C nuclear spin hyperpolarization can increase the sensitivity of detection in an MRI experiment by more than 10,000-fold. (13)C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized (13)C label exchange between injected [1-(13)C]pyruvate and the endogenous tumor lactate pool can be used clinically to assess tumor grade and response to treatment. We describe here an experimental protocol for using this technique in patient-derived and established cell line xenograft models of breast cancer in the mouse. For complete details on the use and execution of this protocol, please refer to Ros et al. (2020).
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spelling pubmed-82204042021-06-28 Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer Ros, Susana Wright, Alan J. Bruna, Alejandra Caldas, Carlos Brindle, Kevin M. STAR Protoc Protocol (13)C nuclear spin hyperpolarization can increase the sensitivity of detection in an MRI experiment by more than 10,000-fold. (13)C magnetic resonance spectroscopic imaging (MRSI) of hyperpolarized (13)C label exchange between injected [1-(13)C]pyruvate and the endogenous tumor lactate pool can be used clinically to assess tumor grade and response to treatment. We describe here an experimental protocol for using this technique in patient-derived and established cell line xenograft models of breast cancer in the mouse. For complete details on the use and execution of this protocol, please refer to Ros et al. (2020). Elsevier 2021-06-17 /pmc/articles/PMC8220404/ /pubmed/34189473 http://dx.doi.org/10.1016/j.xpro.2021.100608 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ros, Susana
Wright, Alan J.
Bruna, Alejandra
Caldas, Carlos
Brindle, Kevin M.
Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer
title Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer
title_full Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer
title_fullStr Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer
title_full_unstemmed Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer
title_short Metabolic imaging with hyperpolarized [1-(13)C] pyruvate in patient-derived preclinical mouse models of breast cancer
title_sort metabolic imaging with hyperpolarized [1-(13)c] pyruvate in patient-derived preclinical mouse models of breast cancer
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220404/
https://www.ncbi.nlm.nih.gov/pubmed/34189473
http://dx.doi.org/10.1016/j.xpro.2021.100608
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