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DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans
Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C. elegans, we identified dyf-4 and daf-6 as two mutants sharing similar defects in den...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221789/ https://www.ncbi.nlm.nih.gov/pubmed/34115759 http://dx.doi.org/10.1371/journal.pgen.1009618 |
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author | Hong, Hui Chen, Huicheng Zhang, Yuxia Wu, Zhimao Zhang, Yingying Zhang, Yingyi Hu, Zeng Zhang, Jian V. Ling, Kun Hu, Jinghua Wei, Qing |
author_facet | Hong, Hui Chen, Huicheng Zhang, Yuxia Wu, Zhimao Zhang, Yingying Zhang, Yingyi Hu, Zeng Zhang, Jian V. Ling, Kun Hu, Jinghua Wei, Qing |
author_sort | Hong, Hui |
collection | PubMed |
description | Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C. elegans, we identified dyf-4 and daf-6 as two mutants sharing similar defects in dendrite extension. DAF-6 encodes a glia-specific patched-related membrane protein that plays vital roles in glial morphogenesis. We cloned dyf-4 and found that DYF-4 encodes a glia-secreted protein. Further investigations revealed that DYF-4 interacts with DAF-6 and functions in a same pathway as DAF-6 to regulate sensory compartment formation. Furthermore, we demonstrated that reported glial suppressors of daf-6 could also restore dendrite elongation and ciliogenesis in both dyf-4 and daf-6 mutants. Collectively, our data reveal that DYF-4 is a regulator for DAF-6 which promotes the proper formation of the glial channel and indirectly affects neurite extension and ciliogenesis. |
format | Online Article Text |
id | pubmed-8221789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82217892021-07-07 DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans Hong, Hui Chen, Huicheng Zhang, Yuxia Wu, Zhimao Zhang, Yingying Zhang, Yingyi Hu, Zeng Zhang, Jian V. Ling, Kun Hu, Jinghua Wei, Qing PLoS Genet Research Article Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C. elegans, we identified dyf-4 and daf-6 as two mutants sharing similar defects in dendrite extension. DAF-6 encodes a glia-specific patched-related membrane protein that plays vital roles in glial morphogenesis. We cloned dyf-4 and found that DYF-4 encodes a glia-secreted protein. Further investigations revealed that DYF-4 interacts with DAF-6 and functions in a same pathway as DAF-6 to regulate sensory compartment formation. Furthermore, we demonstrated that reported glial suppressors of daf-6 could also restore dendrite elongation and ciliogenesis in both dyf-4 and daf-6 mutants. Collectively, our data reveal that DYF-4 is a regulator for DAF-6 which promotes the proper formation of the glial channel and indirectly affects neurite extension and ciliogenesis. Public Library of Science 2021-06-11 /pmc/articles/PMC8221789/ /pubmed/34115759 http://dx.doi.org/10.1371/journal.pgen.1009618 Text en © 2021 Hong et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Hong, Hui Chen, Huicheng Zhang, Yuxia Wu, Zhimao Zhang, Yingying Zhang, Yingyi Hu, Zeng Zhang, Jian V. Ling, Kun Hu, Jinghua Wei, Qing DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans |
title | DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans |
title_full | DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans |
title_fullStr | DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans |
title_full_unstemmed | DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans |
title_short | DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans |
title_sort | dyf-4 regulates patched-related/daf-6-mediated sensory compartment formation in c. elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221789/ https://www.ncbi.nlm.nih.gov/pubmed/34115759 http://dx.doi.org/10.1371/journal.pgen.1009618 |
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