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Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure

INTRODUCTION: The modification of lysine crotonylation (Kcr) is another biological function of histone in addition to modification of lysine acetylation (Kac), which may play a specific regulatory role in diseases. OBJECTIVES: This study compared the expression levels of Kcr and proteome between pat...

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Autores principales: Gan, Qing, Tang, Donge, Yan, Qiang, Chen, Jiejing, Xu, Yong, Xue, Wen, Xiao, Lu, Zheng, Fengping, Xu, Huixuan, Fu, Yingyun, Dai, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221893/
https://www.ncbi.nlm.nih.gov/pubmed/34221209
http://dx.doi.org/10.1155/2021/6652297
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author Gan, Qing
Tang, Donge
Yan, Qiang
Chen, Jiejing
Xu, Yong
Xue, Wen
Xiao, Lu
Zheng, Fengping
Xu, Huixuan
Fu, Yingyun
Dai, Yong
author_facet Gan, Qing
Tang, Donge
Yan, Qiang
Chen, Jiejing
Xu, Yong
Xue, Wen
Xiao, Lu
Zheng, Fengping
Xu, Huixuan
Fu, Yingyun
Dai, Yong
author_sort Gan, Qing
collection PubMed
description INTRODUCTION: The modification of lysine crotonylation (Kcr) is another biological function of histone in addition to modification of lysine acetylation (Kac), which may play a specific regulatory role in diseases. OBJECTIVES: This study compared the expression levels of Kcr and proteome between patients with chronic obstructive pulmonary disease (COPD) combined with type II respiratory failure (RF) to study the relationship between Kcr, proteome, and COPD. METHODS: We tested the Kcr and proteome of COPD combined with type II RF and normal control (NC) using croton acylation enrichment technology and liquid chromatography tandem mass spectrometry (LC-MS/MS) with high resolution. RESULTS: We found that 32 sites of 23 proteins were upregulated and 914 sites of 295 proteins were downregulated. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG), protein domain, and Gene Ontology (GO) analysis on crotonylated protein. In proteomics research, we found that 190 proteins were upregulated and 151 proteins were downregulated. Among them, 90 proteins were both modified by differentially expressed crotonylation sites and differentially expressed in COPD combined with type II RF and NC. CONCLUSION: Differentially expressed crotonylation sites may be involved in the development of COPD combined with type II RF. 90 proteins modified by crotonylation and differentially expressed in COPD combined with type II RF can be used as markers for the study of the molecular pathogenesis of COPD combined with type II RF.
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spelling pubmed-82218932021-07-02 Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure Gan, Qing Tang, Donge Yan, Qiang Chen, Jiejing Xu, Yong Xue, Wen Xiao, Lu Zheng, Fengping Xu, Huixuan Fu, Yingyun Dai, Yong Can Respir J Research Article INTRODUCTION: The modification of lysine crotonylation (Kcr) is another biological function of histone in addition to modification of lysine acetylation (Kac), which may play a specific regulatory role in diseases. OBJECTIVES: This study compared the expression levels of Kcr and proteome between patients with chronic obstructive pulmonary disease (COPD) combined with type II respiratory failure (RF) to study the relationship between Kcr, proteome, and COPD. METHODS: We tested the Kcr and proteome of COPD combined with type II RF and normal control (NC) using croton acylation enrichment technology and liquid chromatography tandem mass spectrometry (LC-MS/MS) with high resolution. RESULTS: We found that 32 sites of 23 proteins were upregulated and 914 sites of 295 proteins were downregulated. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG), protein domain, and Gene Ontology (GO) analysis on crotonylated protein. In proteomics research, we found that 190 proteins were upregulated and 151 proteins were downregulated. Among them, 90 proteins were both modified by differentially expressed crotonylation sites and differentially expressed in COPD combined with type II RF and NC. CONCLUSION: Differentially expressed crotonylation sites may be involved in the development of COPD combined with type II RF. 90 proteins modified by crotonylation and differentially expressed in COPD combined with type II RF can be used as markers for the study of the molecular pathogenesis of COPD combined with type II RF. Hindawi 2021-06-15 /pmc/articles/PMC8221893/ /pubmed/34221209 http://dx.doi.org/10.1155/2021/6652297 Text en Copyright © 2021 Qing Gan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gan, Qing
Tang, Donge
Yan, Qiang
Chen, Jiejing
Xu, Yong
Xue, Wen
Xiao, Lu
Zheng, Fengping
Xu, Huixuan
Fu, Yingyun
Dai, Yong
Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
title Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
title_full Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
title_fullStr Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
title_full_unstemmed Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
title_short Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure
title_sort differential expression study of lysine crotonylation and proteome for chronic obstructive pulmonary disease combined with type ii respiratory failure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8221893/
https://www.ncbi.nlm.nih.gov/pubmed/34221209
http://dx.doi.org/10.1155/2021/6652297
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