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(CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue

This work assesses the potential of new water cluster-based ion beams for improving the capabilities of secondary ion mass spectrometry (SIMS) for in situ lipidomics. The effect of water clusters was compared to carbon dioxide clusters, along with the effect of using pure water clusters compared to...

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Autores principales: Dimovska Nilsson, Kelly, Karagianni, Anthi, Kaya, Ibrahim, Henricsson, Marcus, Fletcher, John S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222020/
https://www.ncbi.nlm.nih.gov/pubmed/33974088
http://dx.doi.org/10.1007/s00216-021-03372-x
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author Dimovska Nilsson, Kelly
Karagianni, Anthi
Kaya, Ibrahim
Henricsson, Marcus
Fletcher, John S.
author_facet Dimovska Nilsson, Kelly
Karagianni, Anthi
Kaya, Ibrahim
Henricsson, Marcus
Fletcher, John S.
author_sort Dimovska Nilsson, Kelly
collection PubMed
description This work assesses the potential of new water cluster-based ion beams for improving the capabilities of secondary ion mass spectrometry (SIMS) for in situ lipidomics. The effect of water clusters was compared to carbon dioxide clusters, along with the effect of using pure water clusters compared to mixed water and carbon dioxide clusters. A signal increase was found when using pure water clusters. However, when analyzing cells, a more substantial signal increase was found in positive ion mode when the water clusters also contained carbon dioxide, suggesting that additional reactions are in play. The effects of using a water primary ion beam on a more complex sample were investigated by analyzing brain tissue from an Alzheimer’s disease transgenic mouse model. The results indicate that the ToF-SIMS results are approaching those from MALDI as ToF-SIMS was able to image lyso-phosphocholine (LPC) lipids, a lipid class that for a long time has eluded detection during SIMS analyses. Gangliosides, sulfatides, and cholesterol were also imaged. [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-021-03372-x.
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spelling pubmed-82220202021-06-28 (CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue Dimovska Nilsson, Kelly Karagianni, Anthi Kaya, Ibrahim Henricsson, Marcus Fletcher, John S. Anal Bioanal Chem Paper in Forefront This work assesses the potential of new water cluster-based ion beams for improving the capabilities of secondary ion mass spectrometry (SIMS) for in situ lipidomics. The effect of water clusters was compared to carbon dioxide clusters, along with the effect of using pure water clusters compared to mixed water and carbon dioxide clusters. A signal increase was found when using pure water clusters. However, when analyzing cells, a more substantial signal increase was found in positive ion mode when the water clusters also contained carbon dioxide, suggesting that additional reactions are in play. The effects of using a water primary ion beam on a more complex sample were investigated by analyzing brain tissue from an Alzheimer’s disease transgenic mouse model. The results indicate that the ToF-SIMS results are approaching those from MALDI as ToF-SIMS was able to image lyso-phosphocholine (LPC) lipids, a lipid class that for a long time has eluded detection during SIMS analyses. Gangliosides, sulfatides, and cholesterol were also imaged. [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-021-03372-x. Springer Berlin Heidelberg 2021-05-11 2021 /pmc/articles/PMC8222020/ /pubmed/33974088 http://dx.doi.org/10.1007/s00216-021-03372-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Paper in Forefront
Dimovska Nilsson, Kelly
Karagianni, Anthi
Kaya, Ibrahim
Henricsson, Marcus
Fletcher, John S.
(CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue
title (CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue
title_full (CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue
title_fullStr (CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue
title_full_unstemmed (CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue
title_short (CO(2))(n)(+), (H(2)O)(n)(+), and (H(2)O)(n)(+) (CO(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and Alzheimer’s model mouse brain tissue
title_sort (co(2))(n)(+), (h(2)o)(n)(+), and (h(2)o)(n)(+) (co(2)) gas cluster ion beam secondary ion mass spectrometry: analysis of lipid extracts, cells, and alzheimer’s model mouse brain tissue
topic Paper in Forefront
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222020/
https://www.ncbi.nlm.nih.gov/pubmed/33974088
http://dx.doi.org/10.1007/s00216-021-03372-x
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